Sequences from confirmation amplicon


Confirmation sequences for line 322H07 and hit At4g14240 are shown.
Line ID 322H07
Locus At4g14240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8206351
Primers used for PCR GC76 - 8474, show primer details
   
Sequence Name 48-K033741-022-322-H07C-8409
GenBank Accession LN506426 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K033741-022-322-H07C-8409
TCTAATCTAACCAAAGCATACAAAAATGGTAATTTCCACTTGATCAACCGTCAGGCATAA
ACAACACATACCAGACTACATTCCAAAACATTCTTGCCCAAACACACTATATTTACCTTG
CCAATGGGAAAGGCAATCGGATAGCAGAGAGTCATTAAAATGCGGACAAGCCAGACAAAA
TTCGCTCCAACCGCGAGTCCATACCTAGTGCATATCGCTTGAGGAATAACCTTAAAAGAC
GAAAACAAATGTTAAACACAGTTCAATCTTTGATATATAACCATAGAGATAGACTAGCAA
AACTAGAGACACCTCCTCCAAGTCCATAAAACACATACCTCACCGAAAGCAAGAACGAAT
GTGACAGAAAGAATAATCGCAACGTATTCATTGAATAACTTATCCAAATATATAGGAAGC
CCCTGCCCAAAATTCCATAAAATCAACTAAGTATAGATCTCCCTCATATTAACCATATAA
CCAAACGACCAACAGACTTTGAATGAAGATAATTACCTCCATAGCCATAGCATTACACAG
AAGCAGTGTCACTAAAAG
   
Sequence Name 58-K033741-022-322-H07C-GC76
GenBank Accession LN509626 [GenBank]
Sequencing primer gene specific primer, GC76: 5'-GCTTTTAGTGACACTGCTTCTGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K033741-022-322-H07C-GC76
CTTCATTCAAGTCTGTTGGTCGTTTGGTTATATGGTTAATATGAGGGAGATCTATACTTA
GTTGATTTTATGGAATTTTGGGCAGGGGCTTCCTATATATTTGGATAAGTTATTCAATGA
ATACGTTGCGATTATTCTTTCTGTCACATTCGTTCTTGCTTTCGGTGAGGTATGTGTTTT
ATGGACTTGGAGGAGGTGTCTCTAGTTTTGCTAGTCTATCTCTATGGTTATATATCAAAG
ATTGAACTGTGTTTAACATTTGTTTTCGTCTTTTAAGGTTATTCCTCAAGCGATATGCAC
TAGGTATGGACTCGCGGTTGGAGCGAATTTTGTCTGGCTTGTCCGCATTTTAATGACTCT
CTGCTATCCGATTGCCTTTCCCATTGGCAAGGTAAATATAGTGTGTTTGGTCAAGAATGT
TTTGGAATGTAGTCTGGTATGTGTTGTTTATGCCTGACGGTTGATCAAGTGGAAATTACC
ATTTTTGTATGCTTTTGGTTAGATTTTAGATTTGGTGCAATGAGTATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGAT
CCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTC
GACGTGTCTACTT

Last Updated on 06.12.2018 16:11