Sequences from confirmation amplicon


Confirmation sequences for line 322H07 and hit At4g16765 are shown.
Line ID 322H07
Locus At4g16765 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9430713
Primers used for PCR 18vf - 8474, show primer details
   
Sequence Name 65-K117751-0022-322-H07C-18vf
GenBank Accession LN511482 [GenBank]
Sequencing primer gene specific primer, 18vf: 5'-CTCTCCAAGCATTTCACATTACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K117751-0022-322-H07C-18vf
ACCTACAAAAACTTTTCCTTTAATAACTCAAAACTTTATCAATTTGTATAGAAATTAGAA
AGAAAGAAACAAGACAAAGCTACCGAGTAACGTTCTTTTCCCACCGGCATCACTCTGTGC
AATGTAGACCTAAACCCATTACAAGAAAATACGAAATGTGAAGAACGCATAAAAACCGCA
CTCACGACTAGGTAAACTGGTTTAAGTTTACCGAAACAATCCATTGGTCCATCTCTCCAT
CATATCACCAATGTTGACTATAAACGCCCTGCGGTTACAGAAAGTCCCCGGATTTCAACA
ATCATTTCTCATCATAAACTAGTAGCTAAGAAAAGCTAGACTTACCCTTTAATTCCAGGG
ACATCTTCCCAAATGTGTGGTTGTTTCGATTTGTCTCTACAAACCTAAGAGTTCAGTTCA
AAGACAGTGTAAGAACATAAGGTTACTAATAGAAGATTATGACGAGAAATGGCTTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCC
   
Sequence Name 40-K117750-0022-322-H07C-8409
GenBank Accession LN503856 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K117750-0022-322-H07C-8409
ATCCTTTCTATTAGTAACCTTATGTTCTTACACTGGTCTTTGAACTGAACTCTTAGGTTT
GTAGAGACAAATCGAAACAACCACACATTTGGGAAGATGTCCCTGGAATTAAAGGGTAAG
TCTAGCTTTTCTTAGCTACTAGTTTATGATGAGAAATGATTGTTGAAATCCGGGGACTTT
CTGTAACCGCAGGGCGTTTATAGTCAACATTGGTGATATGATGGAGAGATGGACCAATGG
ATTGTTTCGGTAAACTTAAACCAGTTTACCTAGTCGTGAGTGCGGTTTTTATGCGTTCTT
CACATTTCGTATTTTCTTGTAATGGGTTTAGGTCTACATTGCACAGAGTGATGCCGGTGG
GAAAAGAACGTTACTCGGTAGCTTTGTCTTGTTTCTTTCTTTCTAATTTCTATACAAATT
GATAAAGTTTTGAGTTATTTAAAGGAAAAGTTTTTTGTAGGTGGTGTTCTTCTTAGATCC
CAATCCAGATTGTAATGT

Last Updated on 06.12.2018 16:11