Sequences from confirmation amplicon


Confirmation sequences for line 323A02 and hit At2g39430 are shown.
Line ID 323A02
Locus At2g39430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16464182
Primers used for PCR KS80 - 8474, show primer details
   
Sequence Name 38-K101501-0022-323-A02C-8409
GenBank Accession LN502918 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K101501-0022-323-A02C-8409
CATTTACAATTGAATATATTCATGCTGGTCATGCTCACCATGTAGCAATACAGTCAGAGA
AAGAGTCTGACTTGTTCCGTCCAGGGAACTGGCCAAGTAAAAGCCTTGAGCTCTCCCTAT
AACGGCTGAGCCAAGCTCGTGGCTTTCGGTTAGCTCATCATCTACAACGGTTATGGTTCC
GAACATGAGATGCTGGAGGGCAGCACCGGGAGGAAGGTTCCCGGCAGTTACAAAAGGGAG
ACTGTTGGCGCTAAGGGCATCGTTGGAATTTCCGTTAGTGTTTTGGATGACGGTGGAGGT
TTGAGCACCGCCNAGTCCGGTGAGAAGTGGAGCTGTGTTTGGGTTAATGACACTGTTGAT
G
   
Sequence Name 38-K101505-0022-323-A02C-KS80
GenBank Accession LN502919 [GenBank]
Sequencing primer gene specific primer, KS80: 5'-ACATCAACAGTGTCATTAACCCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K101505-0022-323-A02C-KS80
GGCTCAACCTCCCCGTCATCCAAACACTAACGGAAATTCCACGATGCCCTTAGCGCCAAC
AGTCTCCCTTTTGTAACTGCCGGGAACCTTCCTCCCGGTGCTGCCCTCCAGCATCTCATG
TTCGGAACCATAACCGTTGTAGATGATGAGCTAACCGAAAGCCACGAGCTTGGCTCAGCC
GTTATAGGGAGAGCTCAAGGCTTTTACTTGGCCAGTTCCCTGGACGGAACAAGTCAGACT
CTTTCTCTGACTGTATTGCTACATGGTGAGCATGACCAGCATGAATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTTCATTCAAAAATGTA

Last Updated on 06.12.2018 16:11