Sequences from confirmation amplicon


Confirmation sequences for line 323A02 and hit At2g39435 are shown.
Line ID 323A02
Locus At2g39435 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16466925
Primers used for PCR 38fr - 8474, show primer details
   
Sequence Name 47-K111327-0022-323-A02C-38fr
GenBank Accession LN506229 [GenBank]
Sequencing primer gene specific primer, 38fr: 5'-ATTACATGAGCGAGAGATAAGGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K111327-0022-323-A02C-38fr
CGTAGACTCCAGATCTTCTGCAGATTGGAGAAAGCGGGTCATCTTCTAGCAGATTGGAGA
AAGCGGGTCATTCTTCAGGTGACTGTACCACATAAATTAAAATAAAACAAAATCTAAAAA
TAATAAATCTGCATATAATAAATCTGACTTAAATTATATATGATAGCCTAAGAAATGGTT
TGTTGTCGTTTAAACTTTTGGGAAATTGAATAAGAATCCAAGTGAGAACGTACGTCGTTT
TCATATTCTTTGCATGTATGATGTATCATATTATATAACGGTCCCATGCCAAAATTATTG
GACTCTTTTAAATTAAATAAACAGTAAATGAAAGAGTATATTCAATTCAAAAAATATGAT
ACATCAGCAATTACCAAATATTTACAATTTTATTGTTGAATATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 39-K111326-0022-323-A02C-8409
GenBank Accession LN503393 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K111326-0022-323-A02C-8409
GCCATTTCATTGAATATATTCAACAATAAAATTGTAAATATTTGGTAATTGCTGATGTAT
CATATTTTTTGAATTGAATATACTCTTTCATTTACTGTTTATTTAATTTAAAAGAGTCCA
ATAATTTTGGCATGGGACCGTTATATAATATGATACATCATACATGCAAAGAATATGAAA
ACGACGTACGTTCTCACTTGGATTCTTATTCAATTTCCCAAAAGTTTAAACGACAACAAA
CCATTTCTTAGGCTATCATATATAATTTAAGTCAGATTTATTATATGCAGATTTATTATT
TTTAGATTTTGTTTTATTTTAATTTATGTGGTACAGTCACCTGAAGAATGACCCGCTTTC
TCCAATCTGCTAGAAGATGACCCGCTTTCTCCAATCTGCTAGAAGATCTTGGAGTCTTAC
GCGTTTCTTTCTCCCTTTTCTCGCTTTCCCTTATCTCTCGTCCATGTAA

Last Updated on 06.12.2018 16:11