Sequences from confirmation amplicon


Confirmation sequences for line 323F09 and hit At3g47720 are shown.
Line ID 323F09
Locus At3g47720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17593321
Primers used for PCR 35sm - 8474, show primer details
   
Sequence Name 60-K116549-0022-323-F09C-35sm
GenBank Accession LN510351 [GenBank]
Sequencing primer gene specific primer, 35sm: 5'-TCTCCAGTTATACTTCTCAGGCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K116549-0022-323-F09C-35sm
CAAGGAACCAACATGAGAATATATTATGATGAAGATGTAAAACAGAGAGCCAAAGAAAGT
GGACTTACTTGATGTTCTTTATAGTGTTTATGAATGAGACGGATTTGCGATTGATTTAGA
AACTTTGATATCGAGTTGATTAAGACCGGAAACGAAATCCAAGGAGATTTCGGGTTTTCT
ATATCAAATAAACATACCAAATCAGCAACTGAATTAAAGAAGAGAGGAACAAACATTTTT
GAGTGCTTACTTCTTTTCAAGATAGATGGAGTTTTGATGCAAACAACAAACTCAGGCAAA
ACATGAGTGTTCATGTGTGTGCTCCAAATAATATACTTGTTTGGAGATGTCAAACTGTCT
ACACCTGAATCAAACTCCATAGAACTCGGATACGATTGTGATGTCGAGCACACAACCTCT
GATTTTCCCATAATAATTCTTGAAAACAGCAAGAAGCTAATCCCGTCTTCGTTACAAGAT
GATGAAGGATCTATCATACTTATACAAAGAAAAAAGAAAACATTATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
   
Sequence Name 20-K116548-0022-323-F09C-8409
GenBank Accession LN495371 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K116548-0022-323-F09C-8409
TTTTCTTTTTTCTTTGTATAGTATGATAGATCCTTCATCATCTTGTAACGAAGACGGGAT
TAGCTTCTTGCTGTTTTCAAGAATTATTATGGGAAAATCAGAGGTTGTGTGCTCGACATC
ACAATCGTATCCGAGTTCTATGGAGTTTGATTCAGGTGTAGACAGTTTGACATCTCCAAA
CAAGTATATTATTTGGAGCACACACATGAACACTCATGTTTTGCCTGAGTTTGTTGTTTG
CATCAAAACTCCATCTATCTTGAAAAGAAGTAAGCACTCAAAAATGTTTGTTCCTCTCTT
CTTTAATTCAGTTGCTGATTTGGTATGTTTATTTGATATAGAAAACCCGAAATCTCCTTG
GATTTCGTTTCCGGTCTTAATCAACTCGATATCAAAGTTTCTAAATCAATCGCAAATCCG
TCTCATTCATAAACACTATAAAGAACATCAAGTAAGTCCACTTTCTTTGGCTCTCTGTTT
TACATCTTCATCATAATATATTCTCATTGTTGGTTCCTTTGCTACTCACAGGATAGGAGA
ATCTCGCGGTGTGAGTTGATTCAACGCCTGAGAAT

Last Updated on 06.12.2018 16:11