Sequences from confirmation amplicon


Confirmation sequences for line 324A03 and hit At5g38420 are shown.
Line ID 324A03
Locus At5g38420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15381779
Primers used for PCR PC65 - 8474, show primer details
   
Sequence Name 71-K103791-0022-324-A03C-PC65
GenBank Accession LN512548 [GenBank]
Sequencing primer gene specific primer, PC65: 5'-TTTCTTAAATTTGCATTGACCACA-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K103791-0022-324-A03C-PC65
ACGCTGATATTCCGAAAGGGGATTAAGCTTCGGTGAAGCTTGGGGGCTTGTAGGCAATGA
AACTGATGCATTGGACTTGACGGGTGTTGTCGAATCCGATGATCCTAATGAAGGCGCCAG
GGTACTCCTTCTTGCATTCTTCAACTTCCTTCAACACTTGAGCGGAGTCGGTGCATCCGA
ACAATGGAAGCTTCCACATTGTCCAGTATCGTCCATCATAGTATCCGGGAGTGTTTCCGT
GCTCACGGTACACAAATCCGTGCTATATATAAAGAAAACCATTCAATACAATTGATATAT
ATATTGGTGATAGTCTATTTCGGTTATCCACTTAAGTTTCTAGCAATGAAGAAGAAGAAT
AAAGGATGCTTAGAGACAAAGGAAAAGTTCAGTGTATATTACCTCCAACTCGAATTCAAC
ACAAGGAATCCACTTGTTGCGGAGAAGGTAGTCAACTTCCTTAGCCAATTCAACGTCACT
AAGGTCAGGGAGGTAAGATAGAGTCTCAAACTTCTTCTTTCCGATTGGTGGCCACACCTA
TNAATTCGAATTCCCAATTATTCTAAAGTATTGTTCAGGATATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCNNAAATGTA
   
Sequence Name 87-K103792-0022-324-A03C-8409
GenBank Accession LN514759 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K103792-0022-324-A03C-8409
GCCATTTACAATTGAATATATCCTGAACATACTTTAGAATAATTGGGAATTCGAATTTAT
AGGTGTGGCCACCAATCGGAAAGAAGAAGTTTGAGACTCTATCTTACCTCCCTGACCTTA
GTGACGTTGAATTGGCTAAGGAAGTTGACTACCTTCTCCGCAACAAGTGGATTCCTTGTG
TTGAATTCGAGTTGGAGGTAATATACACTGAACTTTTCCTTTGTCTCTAAGCATCCTTTA
TTCTTCTTCTTCATTGCTAGAAACTTAAGTGGATAACCGAAATAGACTATCACCAATATA
TATATCAATTGTATTGAATGGTTTTCTTTATATATAGCACGGATTTGTGTACCGTGAGCA
CGGAAACACTCCCGGATACTATGATGGACGATACTGGACAATGTGGAAGCTTCCATTGTT
CGGATGCACCGACTCCGCTCAAGTGTTGAAGGAAGTTGAAGAATGCAAGAAGGAGTACCC
TGGCGCCTTCATTAGGATCATCGGATTCGACAACACCCGTCAAGTCCAATGCATCAGTTT
CATTGCCTACAAGCCCCCAAGCTTCACCGAAGCTTAATCCCCTTTCTGGAATATTCAGCG
TTGATTATTCTGGAACCCATTTCTATGTGGTCAATGCAATTTTAAGAA
Primers used for PCR 88wr - 8474, show primer details
   
Sequence Name 33-K118809-0022-324-A03C-88wr
GenBank Accession LN501195 [GenBank]
Sequencing primer gene specific primer, 88wr: 5'-CCATTTTGAATGAACTCTGTCTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K118809-0022-324-A03C-88wr
GGATGATTCAAGATTATACTTATATTCTTATTATTTAAGATTATCAAGTGGAAAAATAAA
AATATGAATGTGTTAATATAAGGTAATAGAAATTTAATCATTTTTTTAATCTATATGTAA
AAAGTATTTAACCGATATCTACAATTTGACGCCTCCCAATTGAAAGGAGCCAAAAGCAAC
CGATCAAGTGGAGACCAGTAGCCATACACATTCACTCCTACCCTTACATGAGAAAGATAA
GATTATGGAGTTTTCTGCCACGTGATCTTATCCTAGTGGTCCAAATCGATAAGGGTGTCA
ACACCTTTCCTTAATCCTGTGGCAATTAACGACGTTATCATGAATTATGGCCCCTTTGAT
CATTAGGGCTAGTTGCCTCTAGCGGTTCCCACTATATAAAGATGACAAAACCAACAGACA
AACAAGTAAGTAAGAGAAAAACCAAAAGAAGAAGAGAAACAACAAGAAGAAGTAATGGCT
TCCTCTATGTTCTCCTCCACCGCTGTGGTTACCTCCCCGGCTCAAGCCACCATGGTCGCT
CCATTCACCGGCTTGAAGTCATCCGCTTCTTTCCCGGTCACCCGCAAGGCCAACAACGAC
ATTACTTCCATCACAAGCAACGGAGGAAGAGTTAGCTGCATGAAGGTAAATGTCATTAAT
GAAAACTGTCTTTTAAAGCCATTTACAATAGAAACCAACATGCAAGCTCCACCGGGTGCA
AAGCGGCAGCGGCGGNCGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTNCATTCANAAATGT
   
Sequence Name 01-K118808-0022-324-A03C-8409
GenBank Accession LN486794 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K118808-0022-324-A03C-8409
CATTTCATTGAATATATCCTGCCGCCGCTGCCGCTTTGCACCCGGTGGAGCTTGCATGTT
GGTTTCTATTGTAAATGGCTTTAAAAGACAGTTTTCATTAATGACATTTACCTTCATGCA
GCTAACTCTTCCTCCGTTGCTTGTGATGGAAGTAATGTCGTTGTTGGCCTTGCGGGTGAC
CGGGAAAGAAGCGGATGACTTCAAGCCGGTGAATGGAGCGACCATGGTGGCTTGAGCCGG
GGAGGTAACCACAGCGGTGGAGGAGAACATAGAGGAAGCCATTACTTCTTCTTGTTGTTT
CTCTTCTTCTTTTGGTTTTTCTCTTACTTACTTGTTTGTCTGTTGGTTTTGTCATCTTTA
TATAGTGGGAACCGCTAGAGGCAACTAGCCCTAATGATCAAAGGGGCCATAATTCATGAT
AACGTCGTTAATTGCCACAGGATTAAGGAAAGGTGTTGACACCCTTATCGATTTGGACCA
CTAGGATAAGATCACGTGGCAGAAAACTCCATAATCTTATCTTTCTCATGTAAGGGTAGG
AGTGAATGTGTATGGCTACTGGTCTCCACTTGATCGGTTGCTTTTGGCTCCTTTCAATTG
GGAGGCGTCAAATTGTAGATATCGGTTAAATACTTTTTACATATAGATTAAAAAAATGAT
TAAATTTCTATTACCTTATATTAACACATTCATATTTTTATTTTTCCACTTGATAATCTT
AAATAATAAGAATATAAGTATAATCTTTGAATTCATCCCACATTTTAAAAGTATAATTAA
ATCAGACAGAG

Last Updated on 06.12.2018 16:11