Sequences from confirmation amplicon


Confirmation sequences for line 324B04 and hit At3g16480 are shown.
Line ID 324B04
Locus At3g16480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5599948
Primers used for PCR 44ra - 8474, show primer details
   
Sequence Name 29-K115806-0022-324-B04C-44ra
GenBank Accession LN499431 [GenBank]
Sequencing primer gene specific primer, 44ra: 5'-ACAGGAAGTTGATGTTGGGATTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K115806-0022-324-B04C-44ra
TCAGACCATTTGGAAAGTAGTGGTCTTAAGTTTGCTAGGCTCAACATGGTCAGAGAGTGG
TGGTGGAAGAGAGACACCAGCCAAGGGAATGTTCATAGAAGGAAGTGAACTAGAGTAGCC
TCCACTTAGCCAGCTGGAGGAAGAAGAGCTCGTTGCAACAGCACTAGAACTTGCATAACG
AGAAGCTCGAAAATTGTGGTTTAGAATGCCCTATAAACACACACACACACACACACATCC
TAAGCATCATTAATCTCTTGATTCAATTAACATTGTAAAAATAAGAACTTTCGAAACAGA
GACATTTCATAAACCTGCAAAAAGCATGAATAATGGCACCAGCTACTATAAAGCGATAAA
ATTGAATCAAGATCTATAAGAATCTAAGAGACCCTTTAATCCAAATCCAAAAACAATGAA
TCATACAAATTGTTCAATACTAAGCATTCTCCTAAAAAAACCAGAATCTCAATGGAAACA
AGTGAAGTAGGACATTCCCATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCA
   
Sequence Name 08-K115805-0022-324-B04C-8409
GenBank Accession LN489873 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K115805-0022-324-B04C-8409
GAATGTCCTACTTCACTTGTTTCCATTGAGATTCTGGTTTTTTTAGGAGAATGCTTAGTA
TTGAACAATTTGTATGATTCATTGTTTTTGGATTTGGATTAAAGGGTCTCTTAGATTCTT
ATAGATCTTGATTCAATTTTATCGCTTTATAGTAGCTGGTGCCATTATTCATGCTTTTTG
CAGGTTTATGAAATGTCTCTGTTTCGAAAGTTCTTATTTTTACAATGTTAATTGAATCAA
GAGATTAATGATGCTTAGGATGTGTGTGTGTGTGTGTGTGTTTATAGGGCATTCTAAACC
ACAATTTTCGAGCTTCTCGTTATGCAAGTTCTAGTGCTGTTGCAACGAGCTCTTCTTCCT
CCAGCTGGCTAAGTGGAGGCTACTCTAGTTCACTTCCTTCTATGAACATTCCCTTGGCTG
GTGTCTCTCTTCCACCACCACTCTCTGACCATGTTGAGCCTAGCAAACTTAAGACCACTA
CTCTCCCAAATGGTCTGACTATCGCCACTGAGATGTCTCCGGTGAGAGCTTCTTTTAGTA
ATCCCAACATCAACTTCC

Last Updated on 06.12.2018 16:11