Sequences from confirmation amplicon


Confirmation sequences for line 324B04 and hit At5g08660 are shown.
Line ID 324B04
Locus At5g08660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2817072
Primers used for PCR CL85 - 8474, show primer details
   
Sequence Name 56-K032927-022-324-B04C-8409
GenBank Accession LN509014 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K032927-022-324-B04C-8409
TTACAATTGAATAATCCCGAGCAACGCAGACACTTCAGAGTTGGCCTACACTACAATTTT
GACTTTGATTCTGAATACACTGCATTGGCTTGTCCCTGTAACAACAAAGTAAGCATCTGA
CCCTTCTTTCGTCATTTTGACTTTACAATTTTTGATCTCGGTATATAGTTTTATCTTTAA
TGGCTAATGATTGTTGAATGGGTTCACAGAGCGCATCATGGTTTTGGCTGGGTTGGAGAG
TGGGCAAATACAGGGTGAGTGATGAATGAAATTAGACCTGCAACTTCTACATTTTGTCTT
TTTATTTCTCCAATGCTTTCGTTTTTAATCTTACAGGACTGATTTCACTAGTAAGCCTAG
TGGTGGAGATATACTACGTATTGAAACACTCTATCACGCTAGTAAAGAAAAGACAGAGAT
CTACATTCTTGGACAGATAATTTGGTTACAACATTTGGTTACAAAAGCTAAGAGCGACGC
TCGAGGAGGTCCTAGACTTTCTTCTATCAAGTCTCCATTGGATACTACGAACCAGCAATT
GATATCTGAACCACTCAGTGTCCCAATAGTAACAGATGAGG
   
Sequence Name 56-K032927-022-324-B04C-CL85
GenBank Accession LN509015 [GenBank]
Sequencing primer gene specific primer, CL85: 5'-AACATCTTTTGCTCCTCATCTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K032927-022-324-B04C-CL85
CATTGCTGGTTCGTANTATCAATGGAGACTTGATAGAAGAAAGTCTAGGACCTCCTCGAG
CGTCGCTCTTAGCTTTTGTAACCAAATGTTGTAACCAAATTATCTGTCCAAGAATGTAGA
TCTCTGTCTTTTCTTTACTAGCGTGATAGAGTGTTTCAATACGTAGTATATCTCCACCAC
TAGGCTTACTAGTGAAATCAGTCCTGTAAGATTAAAAACGAAAGCATTGGAGAAATAAAA
AGACAAAATGTAGAAGTTGCAGGTCTAATTTCATTCATCACTCACCCTGTATTTGCCCAC
TCTCCAACCCAGCCAAAACCATGATGCGCTCTGTGAACCCATTCAACAATCATTAGCCAT
TAAAGATAAAACTATATACCGAGATCAAAAATTGTAAAGTCAAAATGACGAAAGAAGGGT
CAGATGCTTACTTTGTTGTTACAGGGACAAGCCAATGCAGTGTATTCAGAATCAAAGTCA
AAATTGTAGTGTAGGCCAACTCTGAAGTGTCTGCGTTGCTCGGGATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTT
ATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGG
CGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCC

Last Updated on 06.12.2018 16:11