Sequences from confirmation amplicon


Confirmation sequences for line 324E06 and hit At4g25230 are shown.
Line ID 324E06
Locus At4g25230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12927937
Primers used for PCR 15vh - 8474, show primer details
   
Sequence Name 51-K117914-0022-324-E06C-15vh
GenBank Accession LN507628 [GenBank]
Sequencing primer gene specific primer, 15vh: 5'-CGTGTCTTTATGACTCACACTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K117914-0022-324-E06C-15vh
ATCAGGTCTAATGAGGTTTATTCTTGTCCTACATGTCGTAAGCCTCTTTTCGTTGGTAGA
ACTGAAAATGAGGTGAACCCTCGCACAGTGGAAGTCTCAAGTGATGAGCAGTTAGCCCGT
CAGCTTGAAAGACAAAACAATCCTGTACATGCACTAGCCACTGGATTATTTCCTGCTGAG
GTGCCAGATTCTGTCGAAAATGATACTTCGAGGTTCAGTTCATTCTTCGTTCTTGATTTT
CTTATTAACTACACCAAAGTAACAATTTATTTAGAACTTCATGATTCTTTTCCTGCGACT
CCCTTGATCTTGGTACTGAGATACTAAATTTGCACTGCCTTAAAGTAACGTTATTTTAAA
ATGTTTTGAATAGCAAGTTATATTTGGTTTCTGACGACCACTATTTGTGCATATTTCTTG
GATTTATTTTACTGGATTGTATAAGTTCTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 11-K117913-0022-324-E06C-8409
GenBank Accession LN491310 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K117913-0022-324-E06C-8409
AGAACTTATACATCCAGTAAAATAAATCCAAGAAATATGCACAAATAGTGGTCGTCAGAA
ACCAAATATAACTTGCTATTCAAAACATTTTAAAATAACGTTACTTTAAGGCAGTGCAAA
TTTAGTATCTCAGTACCAAGATCAAGGGAGTCGCAGGAAAAGAATCATGAAGTTCTAAAT
AAATTGTTACTTTGGTGTAGTTAATAAGAAAATCAAGAACGAAGAATGAACTGAACCTCG
AAGTATCATTTTCGACAGAATCTGGCACCTCATCAGGAAATAATCCAGTGGCTAGTGCAT
GTACAGGATTGTTTTGTCTTTCAAGCTGACGGGCTAACTGCTCATCACTTGAGACTTCCA
CTGTGCGAGGGTTCACCTCATTTTCAGTTCTACCAACGAAAAGAGGCTTACGACATGTAG
GACAAGAATAAACCTCATTTATACCTTGATCCAACCTGCAGGAATCAAAACATCACCAGG
ATCACCAGTGTG

Last Updated on 06.12.2018 16:11