Genotyping and primer details


Genotyping and primer details for 324E06 - At4g25230
Line ID 324E06
Locus At4g25230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:12927937 of line 324E06 Chr4:12927937
   
Insertion allele amplicon 8474 - 15vh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 15vh: 5'-CGTGTCTTTATGACTCACACTGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:12927430
   
wt amplicon (over InsPos) 25vu - 15vh
wt amplicon start Chr4:12927430
wt amplicon end Chr4:12928485
wt amplicon size 1056 bp
Locus specific primer 15vh: 5'-CGTGTCTTTATGACTCACACTGGT-3'
2nd locus specific primer 25vu: 5'-ACTGATAAAATTCGGCTTCAGGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11