Sequences from confirmation amplicon


Confirmation sequences for line 324H01 and hit At1g71770 are shown.
Line ID 324H01
Locus At1g71770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26992275
Primers used for PCR 24bk - 8474, show primer details
   
Sequence Name 21-K107548-0022-324-H01C-24bk
GenBank Accession LN495689 [GenBank]
Sequencing primer gene specific primer, 24bk: 5'-AAACACGTGAAAACATCAAGACAG-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K107548-0022-324-H01C-24bk
GAGTTTGATGTTCAAGAGTGAGAGGGCTAAAAAGAACCTGAAGGTGAGCCTGTCTTTCTT
CCTTACGTTGTGCCAAAGCAACGTAAAGAGGTTTCCTTCCTATCATCTTCCCATTCATTT
CTTTCATCTGCGTAATCATCAACCCCAATGAACATTTTAGTACCAATTTTATAAAACATT
TATGTATATGTAAAAAGATTGTCAATTCAGTTGAAACTTACAGCTAATAAAGCTTCTTCA
GGATTGGAGTAAGCAACAAAACCAAAACCTCTGCTTAAACCTTGTGAATTCATCATAACC
TAATTATGACCACAAACAAAACATTTTAATTCATTATATTGTGTGGTATAAACTTATGTA
TAAAGTTTAGAGATGTTTAGAATCAGAAACAAACCTTGCAGGAAGTCACATTCCCATACT
CAGAGAACATCTCCTTGAGCTTCTCATCATTCACACTATCATCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATTA
   
Sequence Name 05-K107547-0022-324-H01C-8409
GenBank Accession LN488382 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K107547-0022-324-H01C-8409
GAGCCATTTACATTGATGATAGTGTGAATGATGAGAAGCTCAAGGAGATGTTCTCTGAGT
ATGGGAATGTGACTTCCTGCAAGGTTTGTTTCTGATTCTAAACATCTCTAAACTTTATAC
ATAAGTTTATACCACACAATATAATGAATTAAAATGTTTTGTTTGTGGTCATAATTAGGT
TATGATGAATTCACAAGGTTTAAGCAGAGGTTTTGGTTTTGTTGCTTACTCCAATCCTGA
AGAAGCTTTATTAGCTGTAAGTTTCAACTGAATTGACAATCTTTTTACATATACATAAAT
GTTTTATAAAATTGGTACTAAAATGTTCATTGGGGTTGATGATTACGCAGATGAAAGAAA
TGAATGGGAAGATGATAGGAAGGAAACCTCTTTACGTTGCTTTGGCACAACGTAAGGAAG
AAAGACAGGCTCACCTTCAGGTTCTTTTTAGCCCTCTCACTCTTTGAACATCAAACTCTG
TACTGTGTTGTGTTGTGACACTGAGTTCTTAAACTGTCTTGATGTTTTCACGTGTT

Last Updated on 06.12.2018 16:11