Sequences from confirmation amplicon


Confirmation sequences for line 325A04 and hit At4g39660 are shown.
Line ID 325A04
Locus At4g39660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18406790
Primers used for PCR AC18 - 8474, show primer details
   
Sequence Name 20-K027789-022-325-A04C-AC18
GenBank Accession LN495069 [GenBank]
Sequencing primer gene specific primer, AC18: 5'-TGATGATCCTTCGTCAAGTGATAG-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K027789-022-325-A04C-AC18
ATTGTGATAATCTTGAAACCTATATAGTACTATTTCATATTTTATATACAATGTTAATTA
GCTCGGAGTTAGGTCCACTAACTAAGTCTAATTACTAAAATAATACAATCTTCTAACTTA
ACAACTAATCATGGTGCACATGCAAAATTAAAATTAAAAAAAAAGATTTGGAGGATTAAT
ACTCCAAAGTCCAAACCTTGGATACTCCGCCTCTAGTCAGTGACACGTCATATAATTAGC
AGACGATAATCGGAAGAGATTTGTTACAGTGTCACTGTCGGTTGCTTAATCAGCAATCTC
ATTCAAATGGAATCTTAATACATGTAGGAATCTTTTTCCTAAGAATCATATTTGACACGT
CATCATTTCTTAAAAATCTTTTGATCCTCCTACTTAAGTAGCACTAATCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
ATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGA
AAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACT
   
Sequence Name 19-K027789-022-325-A04C-8409
GenBank Accession LN494616 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K027789-022-325-A04C-8409
AGCCATTTACATTGATTAGTGCTACTTAAGTAGGAGGATCAAAAGATTTTTAAGAAATGA
TGACGTGTCAAATATGATTCTTAGGAAAAAGATTCCTACATGTATTAAGATTCCATTTGA
ATGAGATTGCTGATTAAGCAACCGACAGTGACACTGTAACAAATCTCTTCCGATTATCGT
CTGCTAATTATATGACGTGTCACTGACTAGAGGCGGAGTATCCAAGGTTTGGACTTTGGA
GTATTAATCCTCCAAATCTTTTTTTTTAATTTTAATTTTGCATGTGCACCATGATTAGTT
GTTAAGTTAGAAGATTGTATTATTTTAGTAATTAGACTTAGTTAGTGGACCTAACTCCGA
GCTAATTAACATTGTATATAAAATATGAAATAGTACTATATAGGTTTCAAGATTATCACA
ATTTTCACATTTGGTCTTAGACGTGTACATGACTATCACTTGA
Primers used for PCR AC19 - 8474, show primer details
   
Sequence Name 33-K027791-022-325-A04C-8409
GenBank Accession LN500860 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K027791-022-325-A04C-8409
ATTGGTACTAAAAATGGCGTTACAAAGGCAACTCCTGAAGAGAGCTACTTCCGATATCTA
TCACCGCCGTGCGATCTCTCTCCTCCGTACTGATTTCTCTACTTCTCCTTCGATTGCTGA
TGCTCCGCCGCACATTCCGCCGTTCGTTCACCAGCCACGGCCGTATAAAGGTCCTTCCGC
CGATGAGGTTTTACAGAAACGGAAGAAGTTTCTCGGACCTTCTCTTTTCCACTACTACCA
GAAGCCTGTACGTCCGTTTATGTTTTCGTTTTCTCGTTCTGCTGATACCGAATTGAGATT
TTGTTATATTGATTTTGGCGATCGCGATTTATCAGTACGCAATCGGGGATTTCAAATTTG
TAGTTGTGATTTTCGATCGATTCATTAGCTCCCGAAGACTA
   
Sequence Name 34-K027791-022-325-A04C-AC19
GenBank Accession LN501279 [GenBank]
Sequencing primer gene specific primer, AC19: 5'-GTCCACAAATCCTCTTAGTCTTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K027791-022-325-A04C-AC19
GAATCCACTACAATTTGAATCCCCGATTGCGTACTGATAAATCGCGATCGCCAAAATCAA
TATAACAAAATCTCAATTCGGTATCAGCAGAACGAGAAAACGAAAACATAAACGGACGTA
CAGGCTTCTGGTAGTAGTGGAAAAGAGAAGGTCCGAGAAACTTCTTCCGTTTCTGTAAAA
CCTCATCGGCGGAAGGACCTTTATACGGCCGTGGCTGGTGAACGAACGGCGGAATGTGCG
GCGGAGCATCAGCAATCGAAGGAGAAGTAGAGAAATCAGTACGGAGGAGAGAGATCGCAC
GGCGGTGATAGATATCGGAAGTAGCTCTCTTCAGGAGTTGCCTTTGTAACGCCATTTATT
AGTTACCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATC
CGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCG
ACGTGTCTAC

Last Updated on 06.12.2018 16:11