Sequences from confirmation amplicon


Confirmation sequences for line 325C01 and hit At3g45590 are shown.
Line ID 325C01
Locus At3g45590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16733533
Primers used for PCR BV31 - 8474, show primer details
   
Sequence Name 85-K105954-0022-325-C01C-BV31
GenBank Accession LN514548 [GenBank]
Sequencing primer gene specific primer, BV31: 5'-GCAGCTTATAGGAATGTCAAGAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K105954-0022-325-C01C-BV31
GAGACAAAATCACATCCTAGTCATTTCCATCTTTGATCAAAATTTGGAACAGTTCAAAAA
AATGTATCGAGGCTTTGATTTATGTTTAAAGGTTTTAGTACTTTTGTGTATGTTTTTGGT
ATGTAACACATTTTTTTGTGTGTATATTATTTATGTTGGAATCGGAACCTAGTTCATTTT
ATATTCTTCTAGAAATCAGCATTACAACAAGACTTGTTAACATGTAAGCTTAATCATGTT
CTGGTTTCATCTTCACGGCTAAGTTCTGGACTCCATCTACGTATTGTTTGCTCTTCAACT
GTGTAGTCCTCTAAACACAAAGGCAGATTCATCTTCTCTGTGTTGACTTTTCTGTTGACG
TAAAGAACCAACAATGATTTCGCGACGCTTCCGGTTAGTCGAACACTGCAATGGATATCG
GACCACACCTTTAACCGATCATTCCCGCCAATGGACTGAACAAGAACGGCGTATTCCAAA
ATATATCCNAAGAAATGACGAANATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATT
   
Sequence Name 21-K105955-0022-325-C01C-8409
GenBank Accession LN495664 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K105955-0022-325-C01C-8409
GAGCCATTTACAATTGAATATATTCGTCATTTCTTTGGATATATTTTGGAATACGCCGTT
CTTGTTCAGTCCATTGGCGGGAATGATCGGTTAAAGGTGTGGTCCGATATCCATTGCAGT
GTTCGACTAACCGGAAGCGTCGCGAAATCATTGTTGGTTCTTTACGTCAACAGAAAAGTC
AACACAGAGAAGATGAATCTGCCTTTGTGTTTAGAGGACTACACAGTTGAAGAGCAAACA
ATACGTAGATGGAGTCCAGAACTTAGCCGTGAAGATGAAACCAGAACATGATTAAGCTTA
CATGTTAACAAGTCTTGTTGTAATGCTGATTTCTAGAAGAATATAAAATGAACTAGGTTC
CGATTCCAACATAAATAATATACACACAAAAAAATGTGTTACATACCAAAAACATACACA
AAAGTACTAAAACCTTTAAACATAAATCAAAGCCTCGATACATTTTTTTGAACTGTTCCA
AATTTTGATCAAAGATGGAAATGACTAGGATGTGATTTTGTCATCCTAGCTTCACCAGCC
GGTTCATCGTTATCGATCCTCTTGACATTCCTATNAGCTG

Last Updated on 06.12.2018 16:11