Sequences from confirmation amplicon


Confirmation sequences for line 325E06 and hit At5g17820 are shown.
Line ID 325E06
Locus At5g17820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5889227
Primers used for PCR FP91 - 3144, show primer details
   
Sequence Name 26-K033043-022-325-E06C-FP91
GenBank Accession LN497814 [GenBank]
Sequencing primer gene specific primer, FP91: 5'-TGTTTGTGTGCTAGCTAGACTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K033043-022-325-E06C-FP91
TGATTTAATGTATTATCCTTCTAAATTTTCAATTTTCAATTTAAATAGGATATTAATATA
TTACTATTATTTTTTATTTTAAGTAGGATATATTTAATCACAAACTAAACAAAAATTGTA
AATATATGAAATGATCTAAAATAGTAATGATTGGTCTTAAGCGAATTTTCATATCATTTT
GTCTATATCATCTTATTTTAATATTTTTATGTTCGGATAGGGCTGTGACGCTTCTCTCCT
CATTGATTCAACCAATTCCGAGAAAACTGCTGGACCAAACGGAAGCGTCAGGGAATTTGA
CCTGATAGACCGGATCAAGGCTCAGCTAGAAGCTGCATGCCCTTCCACAGAAACACTGAT
AGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAAT
GAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCAT
CCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGT
CTTCTTTTTCCACGATGCTCCTCGTGGGTGG
   
Sequence Name 58-K033043-022-325-E06C-3144
GenBank Accession LN509619 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K033043-022-325-E06C-3144
CCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACC
CGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTCTGTGGAAGGG
CATGCAGCTTCTAGCTGAGCCTTGATCCGGTCTATCAGGTCAAATTCCCTGACGCTTCCG
TTTGGTCCAGCAGTTTTCTCGGAATTGGTTGAATCAATGAGGAGAGAAGCGTCACAGCCC
TATCCGAACATAAAAATATTAAAATAAGATGATATAGACAAAATGATATGAAAATTCGCT
TAAGACCAATCATTACTATTTTAGATCATTTCATATATTTACAATTTTTGTTTAGTTTGT
GATTAAATATATCCTACTTAAAATAAAAAATAATAGTAATATATTAATATCCTATTTAAA
TTGAAAATTGAAAATTTTAGAAGGATAATAACATTTAAATTCACTTTGCACCTCCATTAT
TTTTGGACCAGTCTAG
Primers used for PCR 59wv - 8474, show primer details
   
Sequence Name 57-K120400-0022-325-E06C-59wv
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 59wv: 5'-CTTAGGTCTAAGGATCGGATCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K120400-0022-325-E06C-59wv
TTTTTTTTTAACATTTTGAAATTATCATTAATTAAGGATAAAACGTGTTTTTTTTTCCCG
GAAAAAAAGGTTCAATAGGGAAAATAATTTTTTAAAAATAAAATAAGGTTGAATTTTTTT
GAAATATAGAAAACTATCCATATTTTTTTGGTTAAGGACAAAATTTCCCAAAAAAATTTA
TAAAACCCAAAGGTATTTTATAAAAAAATTTGCTAACCGAAAAAAAAAATTATTTTTTGA
CCCTGGGGGGGGCGTAGGATTGAACCAGGACCGGTTTAAAATTCCCGGGAAACCAAAGGG
GGGTCCAGGGACGGGCCCGGCCGCCCATTTCCTGGGAACCTAGGGATTCTGCCCCTAAAA
AAACCCCAATTTCCTTGACCAACAGGGGGGGCCCCCAAAAAACCTCCGGCACCAAAGGGG
TTCACCCCTTTGTCCGGAAAAAAACCCCCGGCCCCAAAAACGGAAATCTTTGGACCCGGT
AAGGTTACTTCAAAATTGTTTGAAACCCTACCGTCCCCCCTTCCCGGGGGGATGCTGTAC
CTGGGGCCCCCGGCAAAGGCCCCCAAGTCCCGGGGGAGGGGAAATCAAACCGGGCCTTTT
GCGGGCCTTTTGGGACCAATTTTTTTTCATTTCAAAAATGTAAATTCCCCACGGAAAAAA
AAA
   
Sequence Name 33-K120399-0022-325-E06C-8474
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K120399-0022-325-E06C-8474
GGCCAGCAAAGGCCAGGTATGATTACCCTTCACACGTGACTCGGTGGCCTTAGCCGGAGG
CCCAAGCTACAGCATCCCCACGGGAAGGCGTGACGGTAGGGTCTCAAACAATCTTGATGT
AACCTTACCGGGTCCAACGATCTCCGTCTCTGGAGCCGTGAGTTTATTCACGAACAAAGG
GATGAACACGTTCGATGCAGTAGCTCTTTTGGGTGCACACACTGTTGGTCAAGGAAATTG
TGGTCTCTTTAGTGACAGAATCACTAGCTTCCAAGGAACTGGACGACCGGACCCGTCCAT
GGACCCCGCTTTGGTTACCAGGTAATTTTAAACCGGTCATGGTTCAATCATACGCCACCA
CTAGAGTCAAAAATTAATTTTATCTTTCGATTAGCAATTTTTTTTATAAATTACATTCGG
ATTTTATAACTTTTTTTGGAATATTTAGTCATTAACAAAATATATATGTATAGTTTTCTA
TATTTCAAAAATATTCAACTTTATTTTATTTTTAAAATATTATTCTCCATATTGAACATT
ATTTACAGGAAAAAAAAAACAGTTTTATCATTAATTAAGGATAATTTCAAAATGTTAAAA
AAAAAAACAACCTAAAAACCATTTTTTTCTAAGGTTAAACATTTTTGATCC

Last Updated on 06.12.2018 16:11