Sequences from confirmation amplicon


Confirmation sequences for line 325E09 and hit At2g34500 are shown.
Line ID 325E09
Locus At2g34500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14540950
Primers used for PCR GC75 - 8474, show primer details
   
Sequence Name 59-K033741-022-325-E09C-GC75
GenBank Accession LN509916 [GenBank]
Sequencing primer gene specific primer, GC75: 5'-TTCACTTGTGTTACACGATTCCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K033741-022-325-E09C-GC75
TTACACGGCTAGAAATAGAAAACTTAATGTGCTATTAATTACAACTTACCAACTAATTAG
AACTTTCTATATGAACCGGATCTTCTACAGCAAATCACGGTCAATTCAAAATATTCATCT
CTCTCTCTCTCTCCACATGGACAAGACATGATTAAGAAAGTATATAAAAGAAACAGAGCA
GAGAAAGACATTTTGTTCCGGTATACCATTGCAATTTGCAAACTGCTTCAATCCAATTTC
CCTAAACCTCTTAGAAAATTCTGCAAAACCCACCAAACAAAGAACACCAAGAAAGACAAA
AGGCAGGCAAGACTACAAAGCAAAACCTTCTTCTTCTTCTTCTTCTCGTTACTCAAATCA
TTTCCATATATACGCAAAAACCATTAAGACAAGCTAGAAACAAAGAAACATGGTTTTCTC
TGTTTCTATATTTGCCTCTCTTGCACCATACTTAATCTCAGCATTTCTTCTTTTCCTTCT
CGTCGAGCAACTCTCTTACCTTTTCAAGAAACGAAACATCCCTGGCCCTTTCTTCGTCCC
TCCAATCATCGGAAACGCCGTTGCACTCGTTCGTGACCCCACTTCCTTCTGGGACAAGCA
ATCCTCCACGGCGAACATCTCAGGCCTCTCCGCCAACTACCTCATCGGAAAATTCATCGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCG
TTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATA
GGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA
   
Sequence Name 49-K033741-022-325-E09C-8409
GenBank Accession LN506747 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K033741-022-325-E09C-8409
GCCATTTCGATGAATTTTCCGATGAGGTAGTTGGCGGAGAGGCCTGCGATGTTCGCCGTG
GAGGATTGCTTGTCCCAGAAGGAAGTGGGGTCACGAACGAGTGCAACGGCGTTTCCGATG
ATTGGAGGGACGAAGAAAGGGCCAGGGATGTTTCGTTTCTTGAAAAGGTAAGAGAGTTGC
TCGACGAGAAGGAAAAGAAGAAATGCTGAGATTAAGTATGGTGCAAGAGAGGCAAATATA
GAAACAGAGAAAACCATGTTTCTTTGTTTCTAGCTTGTCTTAATGGTTTTTGCGTATATA
TGGAAATGATTTGAGTAACGAGAAGAAGAAGAAGAAGAAGGTTTTGCTTTGTAGTCTTGC
CTGCCTTTTGTCTTTCTTGGTGTTCTTTGTTTGGTGGGTTTTGCAGAATTTTCTAAGAGG
TTTAGGGAAATTGGATTGAAGCAGTTTGCAAATTGCAATGGTATACCGGAACAAAATGTC
TTTCTCTGCTCTGTTTCTTTTATATACTTTCTTAATCATGTCTTGTCCATGTGGAGAGAG
AGAGAGAGATGAATATTTTGAATTGACCGTGATTTGCTGTAGAAGATCCGGTTCATATAG
AAAGTTCTAATTAGTTGGTAAGTTGTAATTAATAGCACATTAAGTTTTCTATTTCTAGCC
GTTGTAAGATCTTCTCTTCTTCCTGCATATCAAAAGAGGAATCGTGTAACACCAAGTGA

Last Updated on 06.12.2018 16:11