Sequences from confirmation amplicon


Confirmation sequences for line 325F01 and hit At1g63110 are shown.
Line ID 325F01
Locus At1g63110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23404850
Primers used for PCR 01tj - 8474, show primer details
   
Sequence Name 46-K116770-0022-325-F01C-01tj
GenBank Accession LN505982 [GenBank]
Sequencing primer gene specific primer, 01tj: 5'-CTACCTTTGAACTGTGAGAGGTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K116770-0022-325-F01C-01tj
TACATAGATCCTGCTCAATATGTACAACTTCAGCTTCTAATAGCCTAAGAATCCAAATTT
ACAGAGGATCAAATCTTAAGATAGACCTGCATATGGTGACATCGATGCCTGCTTCAACCA
ATAACCTTCAGCAACTGAAAATGTTCATACAAAACGAAGAAATGTGAGCTGAATTCGAGA
TCTTAGAGAGCTAATGAGGGAGTGAGAAAGAGAGAGGAGATACGGCGGCGAATGCTGGTG
AGAGGGGTAGAGACCTCCGGACGGGAAGAGAGATTGAGATTTCCCGGGAAGGAGATCAAG
ATCAATCGGAAAGCAATGGAAGATAAAGCCCAAATCCAGAATCGGCTTAGCTGCTTCATT
TTCTGCTCCTCCGCCATTTCTCCGTCGTCTTTTCTTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATG
   
Sequence Name 22-K116769-0022-325-F01C-8409
GenBank Accession LN496276 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K116769-0022-325-F01C-8409
CATTTCNATTGAAGAAAGACGACGGAGAAATGGCGGAGGAGCAGAAAATGAAGCAGCTAA
GCCGATTCTGGATTTGGGCTTTATCTTCCATTGCTTTCCGATTGATCTTGATCTCCTTCC
CGGGAAATCTCAATCTCTCTTCCCGTCCGGAGGTCTCTACCCCTCTCACCAGCATTCGCC
GCCGTATCTCCTCTCTCTTTCTCACTCCCTCATTAGCTCTCTAAGATCTCGAATTCAGCT
CACATTTCTTCGTTTTGTATGAACATTTTCAGTTGCTGAAGGTTATTGGTTGAAGCAGGC
ATCGATGTCACCATATGCAGGTCTATCTTAAGATTTGATCCTCTGTAAATTTGGATTCTT
AGGCTATTAGAAGCTGAAGTTGTACATATTTGAGCAGGATCTATGTATCATGGCTCACCA
TTGCTGCTCTCGGTTCTTGGACCTCTCAAGTCAAAGGTA

Last Updated on 06.12.2018 16:11