Sequences from confirmation amplicon


Confirmation sequences for line 326G03 and hit At3g14950 are shown.
Line ID 326G03
Locus At3g14950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5031958
Primers used for PCR 19we - 8474, show primer details
   
Sequence Name 65-K118393-0022-326-G03C-19we
GenBank Accession LN511492 [GenBank]
Sequencing primer gene specific primer, 19we: 5'-TAATTTTTCACCTTCCTAGAGCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K118393-0022-326-G03C-19we
ATGAAAGAGTGTAGCTTCGCGATCATATCGAAAACCGAGTATGTGAGAAAGAAGCTGGGA
ATGGTTCCACTTTGGGGACACATTCTAGTACTCTTTGAGCATCATCAAGCCGAAGAAGTT
TCAGTAGTGCTTCAGCTTTACACATAGCTAGCTACAAAATTTTTATATATGTAAAACACT
TCATTTATTCATAAAGTAACACAAATCAACACAAGAATCTTTGTTCTTTACCTGAGGAGA
AGAATCAGCTCCAGACGCAATGGCTGCACTTACTTCAGTCAAAACAATACTCCATTCACC
GCGTCTTCTTGCATATGTGCATTTGTTCAAGTGTTTATCTACTTGTTGAAGCATCTTCAC
CACCGTTGGATCTAATGGTTCCTCCACTGAATATAAATGTATCCCTGCATTATCCACATA
ACCTAATCTGCAAACCAAGTAAAGACCAAACTGATTATAGATATCTTTTCTCGACCAACA
TTCTATAATAAAGATTTTGATGTTACCTAAGAAGCAATGAGGCTAAACGGTGATGAGCCC
TTGCAAAGTTTGGATCTAATTTGATAGCTATTTCACATTCATTCAACTGATAGTAAATGG
CTTATCATTTGGTGAAGTTTGGATCTATTTTCATATACTCTTTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
   
Sequence Name 17-K118392-0022-326-G03C-8409
GenBank Accession LN494061 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K118392-0022-326-G03C-8409
TTGAAAGAGTATATGAAAATAGATCCAAACTTCACCAAATGATAAGCCATTTACTATCAG
TTGAATGAATGTGAAATAGCTATCAAATTAGATCCAAACTTTGCAAGGGCTCATCACCGT
TTAGCCTCATTGCTTCTTAGGTAACATCAAAATCTTTATTATAGAATGTTGGTCGAGAAA
AGATATCTATAATCAGTTTGGTCTTTACTTGGTTTGCAGATTAGGTTATGTGGATAATGC
AGGGATACATTTATATTCAGTGGAGGAACCATTAGATCCAACGGTGGTGAAGATGCTTCA
ACAAGTAGATAAACACTTGAACAAATGCACATATGCAAGAAGACGCGGTGAATGGAGTAT
TGTTTTGACTGAAGTAAGTGCAGCCATTGCGTCTGGAGCTGATTCTTCTCCTCAGGTAAA
GAACAAAGATTCTTGTGTTGATTTGTGTTACTTTATGAATAAATGAAGTGTTTTACATAT
ATAAAAATTTTGTAGCTAGCTATGTGTAAAGCTGAAGCACTACTGAAACTTCTTCGGCTT
GATGATGCTCAAAGAGTACTAGAATGTGTCCCCAAAGTGGAACCATTCCCAGCTTCTTTC
TCACATACTCGGTTTTTCGATATGATCGCTGAAGCTTACACATCTNTTGTCAAATCCCAA
ATGGAGTTGGCTCTAGG

Last Updated on 06.12.2018 16:11