Sequences from confirmation amplicon


Confirmation sequences for line 327D05 and hit At5g65120 are shown.
Line ID 327D05
Locus At5g65120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26013358
Primers used for PCR CN50 - 8474, show primer details
   
Sequence Name 42-K104161-0022-327-D05C-CN50
GenBank Accession LN504444 [GenBank]
Sequencing primer gene specific primer, CN50: 5'-AAAACACAGAACCCAATTTTTACG-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K104161-0022-327-D05C-CN50
GGTCTGAGGAACTTACGAGGTGGTTGCTTTCACGAGCCAAGATACCTTCCATGTTACGAA
ATAGAGATGTCCATTGTTCCATGGGATTATCTGTATTTGTGGAGTTGTTGGAGTTTTCAG
ATCCATAAATATCCGTTGGCGTTCCTCTATCGTTCCTTTGGATCAAGCCACTAGCTTCTT
TCTGGCTCTTCTTCAACACTGCATTCAACACAAATACATGAAATTTACCATTGTTATTCG
ACTCTGTGTTTTGCAGTGTAATAAGAACACTTACCAGATAATCGACCTTTGATATCCTGG
TAGTGAAGATCTGACCAGATATATGCAGCTGAAGAAATTCATTACATCCACAAAACTAAG
TCAGATCATGTTATTGAGAATCAACGAGACAGCAATAACAAAAAACCAACACACTAAAGA
ATCAGCCAAACCTGTCAGGCAAAATGAACAAGAGCAAGATTTAAGCTCGCCGTCAGCTTC
CATATGGTGATTAACAATTCCCATACCGTCAACATTCGAACATCTATTCATATTAAACGA
TTTGATAGCCTGAGGATTAGGCACTAACAAACCCTCACTTTCATTATTGTTGCTACTAAA
CAGAGTCATAGCTNAGTCTTCACTAGTAACAGCATCGGTTTGACTGCCACATACATTCAT
GTTCGAGTCTTTCTCACACGTCTCTNTCACAGTATCATCTTTAGAAGGTTCCACATCAAT
ATCTCCGGCATTGGTTTCACCCTGAGATTCTNTGACATCTAANTTCTCAGAATCACAATC
ATAACTNATCTTATCCTCAGAAGAAGAAGCTTCATTAGTATCACTAGTCCTAGTACTCTC
TTTGACCAAGTCGTCAACAACTAAACACAACCTCTGGGAAAACTTAACACCGGAGCCTTC
ACGAGCAGCAGGGCTC
   
Sequence Name 42-K104162-0022-327-D05C-8409
GenBank Accession LN504445 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K104162-0022-327-D05C-8409
TTCGGACTGAGCCATTTACATTGCTGATAATTGGGCTTTCAGCGATTTTGGGAGATTTGC
TACTTAAATCTGGAGATGATTCTGGTAAGAGCCTTGCTGCTCGTGAAGGCTCCGGTGTTA
AGTTTTCCAAGAGGTTGTGTTTAGTTGTTGACGACTTGGTCAAAGAGAGTACTAGGACTA
GTGATACTAATGAAGCTTCTTCTTCTGAGGATAAGATTAGTTATGATTGTGATTCTGAGA
ATTTAGATGTCAAAGAATCTCAGGGTGAAACCAATGCCGGAGATATTGATGTGGAACCTT
CTAAAGATGATACTGTGAAAGAGACGTGTGAGAAAGACTCGAACATGAATGTATGTGGCA
GTCAAACCGATGCTGTTACTAGTGAAGACTTAGCTATGACTCTGTTTAGTAGCAACAATA
ATGAAAGTGAGGGTTTGTTAGTGCCTAATCCTCAGGCTATCAAATCGTTTAATATGAATA
GATGTTCGAATGTTGACGGTATGGGAATTGTTAATCACCATATGGAAGCTGACGGCGAGC
TTAAATCTTGCTCTTGTTCATTTTGCCTGACAGGTTTGGCTGATTCTTTAGTGTGTTGGT
TTTTTGTTATTGCTGTCTCGTTGATTCTCAATAACATGATCTGACTTAGTTTTGTGGATG
TAATGAATTTCTTCAGCTGCATATATCTGGTCAGATCTTCACTACCAGGATATCANAGGT
CGATTATCTGGTAAGTGTTCTTATTACACTGCAAAACACAGAGTCGAATAACAATGGTAA
ATTTCATGTATTTGTGTTGAATGCAGTGTTGAAGAAGAGCCAGAAAGAAGCTAGTGGCTT
GATCCAAAGGAACGATNGAGGAACGCCNACGGATATNTATGGATC

Last Updated on 06.12.2018 16:11