Sequences from confirmation amplicon


Confirmation sequences for line 327H10 and hit At1g24120 are shown.
Line ID 327H10
Locus At1g24120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8529525
Primers used for PCR 16ql - 8474, show primer details
   
Sequence Name 71-K115459-0022-327-H10C-16ql
GenBank Accession LN512610 [GenBank]
Sequencing primer gene specific primer, 16ql: 5'-GCTTTTACGCAAGAAAAAGAAGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K115459-0022-327-H10C-16ql
GAGCAGAGCTGGCAAAGTTTGAGACAGAGTACAGGGAAGTACTTGTGCAGTTCACCGATA
TGACCAGTCGATACGCTCAAGAAATGCAATCGGTTAGTCTTTTATTCATGAAAGTTTATA
AATTTCAAACCACCATTCACAGAAAACGAAGAGAAGATGTGTCATGACTGAGTAAATTAG
ACCAGATTCTATGTGTAAGTTTCTGAAATGGTTAGCTTAGTCTGATTATCTTATGGTCTT
TTTGCAGATCGATGAGCTCTTAAAGCAGAGAAATGAAATTCACTCAGCATACACGACGAT
TCAATTCAAAAATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCACGATTATT
   
Sequence Name 22-K115458-0022-327-H10C-8409
GenBank Accession LN496246 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K115458-0022-327-H10C-8409
GCATTTCATTTTTGATTGAATCGTCGTGTATGCTGAGTGAATTTCATTTCTCTGCTTTAA
GAGCTCATCGATCTGCAAAAAGACCATAAGATAATCAGACTAAGCTAACCATTTCAGAAA
CTTACACATAGAATCTGGTCTAATTTACTCAGTCATGACACATCTTCTCTTCGTTTTCTG
TGAATGGTGGTTTGAAATTTATAAACTTTCATGAATAAAAGACTAACCGATTGCATTTCT
TGAGCGTATCGACTGGTCATATCGGTGAACTGCACAAGTACTTCCCTGTACTCTGTCTCA
AACTTTGCCAGCTCTGCTCTTTTAGTGAGTATCTGAGCTTCTACAGACCTGAGGTCTTCT
TTTTCTGCGGTAAAAG

Last Updated on 06.12.2018 16:11