Sequences from confirmation amplicon


Confirmation sequences for line 327H10 and hit T4O24 are shown.
Line ID 327H10
Locus T4O24 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24975680
Primers used for PCR 08ow - 8474, show primer details
   
Sequence Name 45-K115005-0022-327-H10C-08ow
GenBank Accession LN505629 [GenBank]
Sequencing primer gene specific primer, 08ow: 5'-AAATATAAAGCCCATGAAAACACG-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K115005-0022-327-H10C-08ow
TGACCTCGACACATTCTAATTGCTGAATCAAATGGTCAAATTTTGAATTTAGCGATATAT
TTAACATGTTAAGACATTTTGATCTGAGCATTTAATGACTTCTGACTAAGACAGAATATC
AAACCAAAAATCACCAACTTTTTCTATCCTCGATATTAGCTTTTCTCTTCATTTCCCTTT
GTAATTCAGTTCGAGGATCATCAACATTCAACAAAAAGACAAAATCAGAACTATTTAATG
ATGATGGTTGGGGAAACGATCAGAATATGATCCATAGGGGCCCTATTTTCTTTCTTAACA
CGCCATAGGGAAATGGCTTCAATGTCCGGAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCAGCGTTA
   
Sequence Name 13-K115004-0022-327-H10C-8409
GenBank Accession LN492160 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K115004-0022-327-H10C-8409
CATTTCCTATGGCGTGTTAGAAAGAAATAGGGCCCCTATGGATCATATTCTGATCGTTTC
CCCAACCATCATCATTAAATAGTTCTGATTTTGTCTTTTTGTTGAATGTTGATGATCCTC
GAACTGAATTACAAAGGGAAATGAAGAGAAAAGCTAATATCGAGGATAGAAAAAGTTGGT
GATTTTTGGTTTGATATTCTGTCTTAGTCAGAAGTCATTAAATGCTCAGATCAAAATGTC
TTAACATGTTAAATATATCGCTAAATTCAAAATTTGACCATTTTGATTCAGCAATTAGAA
TGTGTCGAGGTCATCTGGAGCAATCTGGATATGACTCATCTTCGTGTTTTCATGGCTTTT
ATATT

Last Updated on 06.12.2018 16:11