Sequences from confirmation amplicon


Confirmation sequences for line 328G05 and hit At5g38710 are shown.
Line ID 328G05
Locus At5g38710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15502055
Primers used for PCR NS11 - 8474, show primer details
   
Sequence Name 45-K020436-022-328-G05C-8409
GenBank Accession LN505379 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K020436-022-328-G05C-8409
TCCATGTAGATTTCCCGGACATGAAGCCATTTACAAATATCGAATAATCACTTTGTGCAA
GTCGGATCTACAAACAAACCACTATACATGTTTATAGGTGTAGTCGTGTAGAGGCAAATG
GAGAGAAATGAGGTCAATTGACCCCAATAGTTCTTTGCTATGTTTATATTAATTTTCTGT
TGGATAATTTGACACAAAACTACCTCCACCAGTTCGATTCTTCTATATGTTTTCAGATAT
TTTTATTTTTTGAAAAATGATGCCACTAGATTTTTTGTTTTCAATATCTGCCACTCCAAA
AACGTGGAGTAACCGACCAATTATAACCTTTATAAGAAATACAAATATATAATAAATTAA
ATTACAAAAGAGACTACCCCGTTTAATAAACCTTAAAAAACAAAAAAGAACACCAACTTG
TCTGATTTTTTTTTCGATAACGATTTCTTCTTTGTTTGTTTTAAACGACTTGTCTGAATT
TTAGCTTTTTTTTTTTTTTTTTTTTTGGGGGGGAAAAAAAAAAAAAACAATCAGGGCCCC
GGGGGTTCCTCCCAAAATTTAAAATTAA
   
Sequence Name 46-K020436-022-328-G05C-NS11
GenBank Accession LN505727 [GenBank]
Sequencing primer gene specific primer, NS11: 5'-TTGCGTGATTAACGTGTTAACTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K020436-022-328-G05C-NS11
TCTGACGAGGAACCGACGTGACTTTGATTGTTATTTCATATTTACAGACAAAAAAAAAAA
AAAAAAAAAAAACCAAATTTCCCCCCCCCCTTTTAAAACCCCCCAAAAAAAAATTTTTTT
CCAAAAAAAAACCCCCCCCGGGGGGGGTCTTTTTTTTTTTTTAGGGGTTAAAACCCCGGG
GCCCCCCCTTTGGAATTTACTTTTTTAAAATTTTGGTTTTCCTAAAAGGGGTAAAATTGG
CCCGGCCCCCCCCCTTTTTGGGGGGGCCCAATTTTAAACCCAAAAACTCGGGGGCCCTTT
TTTTCCCAAAAAAAAAATTTTTTACCCCCTTTTAAAAAACCCCCCCGGGGGGGGGATTTT
TTGGCCCAATTTCCCCCCCCAAAATTTTTTTACCCCTCCCCCCCAACCTTTGGGGGCCAA
TGCCCCCCTTTCCCCCCCTTTGCCCCCCCCCCCACCCCCCTAAAAACCTTTTAAGGGGTT
TTTTTTGAAACCCCCCTTCCCCCCAGGGGTTTTTCCAATTTTTGAAAGGGGTTTTTGGCC
CGGAAATTCCCCTGGGTCCCCCAAGGAAAATGGGGCCCCATTTGGGAAAAAAAAAAAAAA
AATTCCCCCTTTTTTTTCCTTTCCCAAAGGGAAATTCCCCCCCCCTTTTTTAAAAAGGGC
CCCCCTTTTTTTTAAACCCCCCAATTCCCCCCCCCC

Last Updated on 06.12.2018 16:11