Sequences from confirmation amplicon


Confirmation sequences for line 329B06 and hit At5g35730 are shown.
Line ID 329B06
Locus At5g35730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:13894973
Primers used for PCR PF26 - 8474, show primer details
   
Sequence Name 42-K102871-0022-329-B06C-PF26
GenBank Accession LN504424 [GenBank]
Sequencing primer gene specific primer, PF26: 5'-AAGAGCAGTAGTTGGCCAATAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K102871-0022-329-B06C-PF26
CATATATTCCTAACCACATAATTATTAGAAGTTAGGAAGATCTCTCAAAGAGCTGGTTGA
AATTTAATACCTTTACCATGGAGGTCAGAATATCTGCCAAAAAGAAGTCTGGAAATGTAA
TTGGCTGTGAAACAAAAGAAACAGGATCAGATATTTATGCCTAAATGAATGAATTTATAC
AAAGATACCAAAGTAATGTAAGAGTTTGAACAAACACAAACGTCACTGATCAAGCATAAC
ACTTATAAACCAGCTATTAAGCAAAACCTAGAAATTCGCCAATTGCAAGTTTAATTTTAA
TCAACAGCTGTCTATCACTTTACCTGCAGTGGAAAAGCTATCCTCCATAATGTTCTCAGC
AGAAAATATCGACTTGACAAGTAGAAAATATCGAAAGGAAATATCAGTACCAAAGCAAAA
GCAATGTACAGGAGCACCTGCAGCAATGATAACACAAACCTTAGAGAGCAAAATGCAATT
AACATGGATCTGTGAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAA
ACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTC
GGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAATGG
   
Sequence Name 42-K102876-0022-329-B06C-8409
GenBank Accession LN504425 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K102876-0022-329-B06C-8409
GCCTTTACATTGAATAATTCACAGATCCATGTTAATTGCATTTTGCTCTCTAAGGTTTGT
GTTATCATTGCTGCAGGTGCTCCTGTACATTGCTTTTGCTTTGGTACTGATATTTCCTTT
CGATATTTTCTACTTGTCAAGTCGATATTTTCTGCTGAGAACATTATGGAGGATAGCTTT
TCCACTGCAGGTAAAGTGATAGACAGCTGTTGATTAAAATTAAACTTGCAATTGGCGAAT
TTCTAGGTTTTGCTTAATAGCTGGTTTATAAGTGTTATGCTTGATCAGTGACGTTTGTGT
TTGTTCAAACTCTTACATTACTTTGGTATCTTTGTATAAATTCATTCATTTAGGCATAAA
TATCTGATCCTGTTTCTTTTGTTTCACAGCCAATTACATTTCCAGACTTCTTTTTGGCAG
ATATTCTGACCTCCATGGTAAAGGTATTAAATTTCAACCAGTTCTCTGAGAGATCTTCCT
AACTTCTAATAATTATGTGGTTAGGAATATATTGATAGCAGGAGGCTTAGTATAACTTGT
GCCTTTTTTATTGGCCAACTACTGCTCT

Last Updated on 06.12.2018 16:11