Sequences from confirmation amplicon


Confirmation sequences for line 329D12 and hit At4g24973 are shown.
Line ID 329D12
Locus At4g24973 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12841112
Primers used for PCR 55qv - 8474, show primer details
   
Sequence Name 69-K115779-0022-329-D12C-55qv
GenBank Accession LN512264 [GenBank]
Sequencing primer gene specific primer, 55qv: 5'-TAAAATCTTGTAACAAAGGTTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K115779-0022-329-D12C-55qv
GTACATTTTTCTGATCCGTTACCTACATGGACCACTAGGTCTAATACTCCAAACACAGGG
ATGATATTTACATACAATTGTATCTCTCTTTTCCTTAAGAATATCGAACCATCTAGACTG
ATTTTCCCAAACAAAGTGACAAAAAAACAGAGTCGTCTCCCAAACATTAAGAGATGCAGT
AAATCCCCAGTGTTGATGAGGGGCTAAAGATTGAGGTCCGAGATCTTTACCCTTCGATTT
GCAGTGTAGTCCCAATTGAACATTTGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACAGTTAT
AAT
   
Sequence Name 21-K115778-0022-329-D12C-8409
GenBank Accession LN495799 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K115778-0022-329-D12C-8409
AATCGAAGGTAAGATCTCGGACCTCAATCTTTAGCCCCTCATCAACACTGGGGATTTACT
GCATCTCTTAATGTTTGGGAGACGACTCTGTTTTTTTGTCACTTTGTTTGGGAAAATCAG
TCTAGATGGTTCGATATTCTTAAGGAAAAGAGAGATACAATTGTATGTAAATATCATCCC
TGTGTTTGGAGTATTAGACCTAGTGGTCCATGTAGGTTAACGGATCATGAAAAATGTTAC
CCATGTAATGCTGATATTTGAATGAAAACCTTTGTTACAAGATTTTAA

Last Updated on 06.12.2018 16:11