Sequences from confirmation amplicon


Confirmation sequences for line 330A11 and hit At5g58770 are shown.
Line ID 330A11
Locus At5g58770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23735091
Primers used for PCR 02ya - 8474, show primer details
   
Sequence Name 76-K119624-0022-330-A11C-02ya
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 02ya: 5'-CTGAGTTCCCAAGTCCTGATCTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K119624-0022-330-A11C-02ya
TACTTCTTCTTGTGGCACTTGCTTATACGAGCTCTTCTTCTCGCCGGTCCTTTGGCCTGA
TTTCGATAAGGATAAGCTTCTAGAGGCCCTGGTTTCGTATCAGCGCCGGGAAAGACGATT
TGGCTGTCGGGTTTGATCCTCTGCTCCGCTCTGTTCTGTTATGTTCATCTGAGATTTCTA
TCAAACTACTTGTTCCACAGTCAGAGATCATTATGCTGTTGTAACTTTATGTCGGTAAAT
TGAAAATTTGCTAATAAAAATCAGCGGTTACTTTGTCGTTACTTATCACTAAAACAAATA
TCTTGCAACACAAGGAACTGTACTATCTCAAAAGCATAGTTCTTGAGGTATATTACACAT
CATATTCAGTTTTCATTACACCATTTGCAAGAAAATCAAGATTTACGACCACCAAAGCGT
CGTTGTCTCTGCTGAAACGACATCAAAGCTTCGATAAAACCGCTTCTCCCAAAATCAGGC
CAAAGTTCTTGAGCAAAGAAAAGCTCTGTATAAGCCAATTGCCACAACAAGAAGTTACTC
ACTCTAAGCTCACCACTCGTTCTTATAAGTAAATCCGGGTAAGGAAACTCAGTACAATTC
GTCTCCAATTCTTCCTCAATCAGTCTCTCGTCTATCTCCTCAACTTCGATTTCGCCATCT
TTCACTCTCCTCGCGATACCTCTACACGCTTGTAAAACATCGTATTTCCCACTGTAACCA
ACCGCTACGATTAGCTGAAGCCTCGTGTTGTTCTTTGTAACTTCCTCAACTTCGTTAATC
ACTCTNAAAAGAGATTTCGGGACAATGTTCATTTCATTTAGGAACAATGTTCGGATATNT
ACATGGATCANGCATGAGTATGATGGTCAATATGGAGAAAAAGAAGAGTAATTACCAATT
TTTTTTCAATTCAAAATGT
   
Sequence Name 52-K119623-0022-330-A11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K119623-0022-330-A11C-8409
TCTAATGAATGAACATTGTCCCGAAATCTCTTTTAAGAGTGATTAACGAAGTTGAGGAAG
TTACAAAGAACAACACGAGGCTTCAGCTAATCGTAGCGGTTGGTTACAGTGGGAAATACG
ATGTTTTACAAGCGTGTAGAGGTATCGCGAGGAGAGTGAAAGATGGCGAAATCGAAGTTG
AGGAGATAGACGAGAGACTGATTGAGGAAGAATTGGAGACGAATTGTACTGAGTTTCCTT
ACCCGGATTTACTTATAAGAACGAGTGGTGAGCTTAGAGTGAGTAACTTCTTGTTGTGGC
AATTGGCTTATACAGAGCTTTTCTTTGCTCAAGAACTTTGGCCTGATTTTGGGAGAAGCG
GTTTTATCGAAGCTTTGATGTCGTTTCAGCAGAGACAACGACGCTTTGGTGGTCGTAAAT
CTTGATTTTCTTGCAAATGGTGTAATGAAAACTGAATATGATGTGTAATATACCTCAAGA
ACTATGCTTTTGAGATAGTACAGTTCCTTGTGTTGCAAGATATTTGTTTTAGTGATAAGT
AACGACAAAGTAACCGCTGATTTTTATTAGCAAATTTTCAATTTACCGACATAAAGTTAC
AACAGCATAATGATCTCTGACTGTGGAACAAGTAGTTTGATAGAAATCTCAGATGAACAT
AACAGAACAGAGCGGAGCAGAGGATCAAACCCGACAGCCAAATCGTCTTTCCCGGCGCTG
ATACGAAACCAGGGCCTCTAGAAGCTTATCCTTATCGAAATCAGGCCAAAGGACCGGCGA
GAAGAAGAGCTCTGTATAAGCAAGTTGCCACAAGAAGAAGTTACTAATCCTCTGTTCTCC
ACTTGTCCTAATCAATAGATCA

Last Updated on 06.12.2018 16:11