Sequences from confirmation amplicon


Confirmation sequences for line 331B09 and hit At3g17510 are shown.
Line ID 331B09
Locus At3g17510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5989589
Primers used for PCR MZ48 - 8474, show primer details
   
Sequence Name 09-K033096-022-331-B09C-MZ48
GenBank Accession LN490072 [GenBank]
Sequencing primer gene specific primer, MZ48: 5'-GTTTCAAAGATCTGCTCTCTGCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K033096-022-331-B09C-MZ48
ATCATACTTTTACTTTCTCAGATGTATCAGAGAGGAGAATAAGATTCACTTCAAATAGCT
CAGCAAAAGATTTACTGGAGAAAATCGAAACAGCCGTTACAGAAATGGGATTCAGTGTAC
AAAAGAAACATGCCAAGGTGGGTCTTCACAACATGGTCTTCCTCTTCCTTAGCAACTTGG
AATTTCAAATCTGTAGGTTTATAAACTTTAAACTGTATTTTGTTTCAGTTAAGAGTAAAA
CAAGAAGAAAGAAACCAGAAAGGTCAAGTTGGTTTATCAGTAACAGCTGAGGTATATTAA
CTATTACGTAAACATTATTTCACTGTTCACTGAACGTGGATATATGGTTTACTAAATAGT
CTACAGGATATATTCACTAAATAGTCACTGAACGTGGATATATGGTTTACTAAATAGTCT
ACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATT
ACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT
ATTTCGACGTGTCTA
   
Sequence Name 57-K033096-022-331-B09C-8409
GenBank Accession LN509330 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K033096-022-331-B09C-8409
ATTTACATTGAATATATCCTGTAGACTATTTAGTAAACCATATATCCACGTTCAGTGACT
ATTTAGTGAATATATCCTGTAGACTATTTAGTAAACCATATATCCACGTTCAGTGAACAG
TGAAATAATGTTTACGTAATAGTTAATATACCTCAGCTGTTACTGATAAACCAACTTGAC
CTTTCTGGTTTCTTTCTTCTTGTTTTACTCTTAACTGAAACAAAATACAGTTTAAAGTTT
ATAAACCTACAGATTTGAAATTCCAAGTTGCTAAGGAAGAGGAAGACCATGTTGTGAAGA
CCCACCTTGGCATGTTTCTTTTGTACACTGAATCCCATTTCTGTAACGGCTGTTTCGATT
TTCTCCAGTAAATCTTTTGCTGAGCTATTTGAAGTGAATCTTATTCTCCTCTCTGATACA
TTCTGAGAAAGTAAAAGTATAACTCCTTTTTATTATACATTGGCTCAAGCAAGAGC

Last Updated on 06.12.2018 16:11