Sequences from confirmation amplicon


Confirmation sequences for line 331D10 and hit At3g16100 are shown.
Line ID 331D10
Locus At3g16100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5459538
Primers used for PCR IH20 - 8474, show primer details
   
Sequence Name 76-K033215-022-331-D10C-IH20
GenBank Accession LN513330 [GenBank]
Sequencing primer gene specific primer, IH20: 5'-TTGACATCATTCACAAGAACACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K033215-022-331-D10C-IH20
ACACCCAAACTTTGGACCTCTCTTGCCCTGCAGTATCCCAAATCTACAATGGGAAAACAA
ATTACTTTATTTCTATCTGTATTTGGAGATCAAAGAACCAAAGAGTTATATAACCTAACA
CACTTAAAAGACAATCCATAAAAACTAGAGTATGTATGTAAACTAAAGTGTGTGTTTCTA
AAACTCATCAGTCACATTATATATATAATTAGATAACACACATGGTGATTACCTGCAAAG
TGAAGATTCTGTCATCAATTTGGACCTCTTTGGTCAAGAAATCAGCTCCAATGGTAGCTT
TATACTGATTACTGAATTTTCGATTCACAAAATTGTAAATGGAAATAAAATGGTAGCTCC
AATGTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTAC
GATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTAT
TTCGACGTGTCTACATTCACTCCCAAATGG
   
Sequence Name 68-K033215-022-331-D10C-8409
GenBank Accession LN511941 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K033215-022-331-D10C-8409
TTACAATTGAATATACATTGGAGCTACCATTTTATTTCCATTTACAATTTTGTGAATCGA
AAATTCAGTAATCAGTATAAAGCTACCATTGGAGCTGATTTCTTGACCAAAGAGGTCCAA
ATTGATGACAGAATCTTCACTTTGCAGGTAATCACCATGTGTGTTATCTAATTATATATA
TAATGTGACTGATGAGTTTTAGAAACACACACTTTAGTTTACATACATACTCTAGTTTTT
ATGGATTGTCTTTTAAGTGTGTTAGGTTATATAACTCTTTGGTTCTTTGATCTCCAAATA
CAGATAGAAATAAAGTAATTTGTTTTCCCATTGTAGATTTGGGATACTGCAGGGCAAGAG
AGGTTCCAAAGTTTGGGTGTTGCTTTCTACCGAGGAGCGGACTGTTGTGTTCTTGTGAAT
GATGTCA

Last Updated on 06.12.2018 16:11