Sequences from confirmation amplicon


Confirmation sequences for line 331E02 and hit At1g53270 are shown.
Line ID 331E02
Locus At1g53270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19863357
Primers used for PCR MD09 - 8474, show primer details
   
Sequence Name 05-K024601-022-331-E02C-8409
GenBank Accession LN488189 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K024601-022-331-E02C-8409
CCGGACATGAAGCCATTTACAATTGAATATATCCTGTAACATGTTCTAGACCAAGCTCTT
GAATCAGCCTCTTCACCTTTGCAGCCGCGTCTTTCCTCTTTGTCTTCAGCCTCAAGAGTG
CGCTGTACGTCAGTGTTTCTTGCACCGTAAGAAACGGAAACAGAGCATCTTCTTGTGGCA
CAAACCCTGAAACCCTCCTGTACTCAGGACCATCCATAGGCCTACCGTTGACAAGCACTT
GCCCTGAAACTTTCCCATGAGAAACTTTTCCGGCTAGAATCTCCAGCAACGTGGTTTTCC
CTGCTCCGCTTGGACCCGCGATCGCAGTGATCTCCGCGGATCTTGCATCACAAGAAACAT
CCTTTAAGATAACCTTTTCCTCCTTCTCACTCAACAAACCACATAAATTAGAAAACTTTG
GAGTGTTTCCGCCTATTCTATACGACAGATTCTTGGTTTCTAATCTATATGAGATTTCCC
GGCCACCGGGAATAGGAGCTTTTACCGGCAATTCCATCTAAGAAAGAAAGAAGGAAAAAG
AA
   
Sequence Name 06-K024601-022-331-E02C-MD09
GenBank Accession LN488639 [GenBank]
Sequencing primer gene specific primer, MD09: 5'-CAAAGTTTGATGCTTTCTTTTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K024601-022-331-E02C-MD09
CTTTCTTTCTTAGATGGAATTGCCGGTAAAAGCTCCTATTCCCGGTGGCCGGGAAATCTC
ATATAGATTAGAAACCAAGAATCTGTCGTATAGAATAGGCGGAAACACTCCAAAGTTTTC
TAATTTATGTGGTTTGTTGAGTGAGAAGGAGGAAAAGGTTATCTTAAAGGATGTTTCTTG
TGATGCAAGATCCGCGGAGATCACTGCGATCGCGGGTCCAAGCGGAGCAGGGAAAACCAC
GTTGCTGGAGATTCTAGCCGGAAAAGTTTCTCATGGGAAAGTTTCAGGGCAAGTGCTTGT
CAACGGTAGGCCTATGGATGGTCCTGAGTACAGGAGGGTTTCAGGGTTTGTGCCACAAGA
AGATGCTCTGTTTCCGTTTCTTACGGTGCAAGAAACACTGACGTACAGCGCACTCTTGAG
GCTGAAGACAAAGAGGAAAGACGCGGCTGCAAAGGTGAAGAGGCTGATTCAAGAGCTTGG
TCTAGAACATGTTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGAT
AAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAA
TTCGGAAATCTTT

Last Updated on 06.12.2018 16:11