Sequences from confirmation amplicon


Confirmation sequences for line 332A08 and hit At3g48960 are shown.
Line ID 332A08
Locus At3g48960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18150719
Primers used for PCR GD74 - 8474, show primer details
   
Sequence Name 71-K033709-022-332-A08C-GD74
GenBank Accession LN512499 [GenBank]
Sequencing primer gene specific primer, GD74: 5'-ATCAGCTTGTGGTTAAGTCCTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K033709-022-332-A08C-GD74
TTTGAGTGAGGATTTAACACAGAAGCTAATAGGATGTGTGATAATGATCTTCTCCATAAA
ACAGAGAAGCAAGGTAGCAACAAAATCGAAGATTTAAGGATAAAAAAGCAGAGTGTGATA
CACAAGAATTAAAATCCAAAAGATGACAAAGATAAAAAGTTTCTTCAAGCTATGAAGAAA
GATACTCACTTCTTCTCTTCTTTCTCAGCCTCAGCAGCTCTCTTGGCTCTAACACCAGCA
TGTCTCTTGTTTATGCCCTCTAGACGGATCTTGTCATAAGCATTGAACAATTTCATGTCA
GATGTCAGCTTCACAAGCTCCATTGTTGGCATTTCACGAACGATAGGCATGTGATCTACT
TGGACTTGTGTTGCATTGGCCAACTCTTGTTGTGCAGAATCACCAACCTAAACACAAACA
AAAGCATAACAACAAAGAGATCATATCCAAATCCATTCCATTGAGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGA
AAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAA
ACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT
   
Sequence Name 55-K033709-022-332-A08C-8409
GenBank Accession LN508725 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K033709-022-332-A08C-8409
TCATGGAATGGATTTGGATATGATCTCTTTGTTGTTATGCTTTTGTTTGTGTTTAGGTTG
GTGATTCTGCACAACAAGAGTTGGCCAATGCAACACAAGTCCAAGTAGATCACATGCCTA
TCGTTCGTGAAATGCCAACAATGGAGCTTGTGAAGCTGACATCTGACATGAAATTGTTCA
ATGCTTATGACAAGATCCGTCTAGAGGGCATAAACAAGAGACATGCTGGTGTTAGAGCCA
AGAGAGCTGCTGAGGCTGAGAAAGAAGAGAAGAAGTGAGTATCTTTCTTCATAGCTTGAA
GAAACTTTTTATCTTTGTCATCTTTTGGATTTTAATTCTTGTGTATCACACTCTGCTTTT
TTATCCTTAAATCTTCGATTTTGTTGCTACCTTGCTTCTCTGTTTTATGGAGAAGATCAT
TATCACACATCCTATTAGCTTCTGTGTTAAATCCTCACTCAAACCTTATCCACTCCTAAC
TAAATGTTAAAACTTAAAACAGGACTTAACCCAAAGCTGAT

Last Updated on 06.12.2018 16:11