Sequences from confirmation amplicon


Confirmation sequences for line 332C03 and hit At5g42420 are shown.
Line ID 332C03
Locus At5g42420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16969447
Primers used for PCR BV38 - 8474, show primer details
   
Sequence Name 11-K105916-0022-332-C03C-BV38
GenBank Accession LN491114 [GenBank]
Sequencing primer gene specific primer, BV38: 5'-TGAGAGAAGAATGAATAGCTGCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K105916-0022-332-C03C-BV38
TACTAGCAGAGCCATGAAGAGAGATACTTACAAAGCAACCGGAAGACATATTATACTTCA
TAATGAACTTCCCACTAAGAAGATAATCAACTAAAGGACCGACAACAAGAAGCGAAAAAG
CTTGAATCGGCGCTGTTTTACTCAACAACTCAAAGGACCCAATTGAATATTTCTTCTGTA
GAGAACCTATTAACTAAGCAACACAGAAGGAAAAAACACCAATTGATCAGATTTGAATTC
CACAAGCTTCAAAACCAAATCACCAAACAAAAAGACTTACAATTTGCTGCAAGGATGACG
AGAAAATCGCAACACAAGCACAGATAAAACCTTTGGCATTAACTTTAACATCAGTCACAG
TACAAATACCAACAATATGATAGTCAATCACAGGATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 11-K105917-0022-332-C03C-8409
GenBank Accession LN491115 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K105917-0022-332-C03C-8409
CGGAATGAGCCATTTACAATTGAATATATCCTGTGATTGACTATCATATTGTTGGTATTT
GTACTGTGACTGATGTTAAAGTTAATGCCAAAGGTTTTATCTGTGCTTGTGTTGCGATTT
TCTCGTCATCCTTGCAGCAAATTGTAAGTCTTTTTGTTTGGTGATTTGGTTTTGAAGCTT
GTGGAATTCAAATCTGATCAATTGGTGTTTTTTCCTTCTGTGTTGCTTAGTTAATAGGTT
CTCTACAGAAGAAATATTCAATTGGGTCCTTTGAGTTGTTGAGTAAAACAGCGCCGATTC
AAGCTTTTTCGCTTCTTGTTGTCGGTCCTTTAGTTGATTATCTTCTTAGTGGGAAGTTCA
TTATGAAGTATAATATGTCTTCCGGTTGCTTTGTAAGTATCTCTCTTCATGGCTCTGCTA
GTATTGTTATGTTATCATTGGCTAATGTTCTACAATTTGCAGCTATTCATCNTTCTCTC

Last Updated on 06.12.2018 16:11