Sequences from confirmation amplicon


Confirmation sequences for line 333B07 and hit At3g05170 are shown.
Line ID 333B07
Locus At3g05170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1466760
Primers used for PCR 16us - 8474, show primer details
   
Sequence Name 43-K117387-0022-333-B07C-16us
GenBank Accession LN504983 [GenBank]
Sequencing primer gene specific primer, 16us: 5'-AAACAAAAGAGAGATGGTGGTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K117387-0022-333-B07C-16us
ATCAATTGACCTTTTATCTCTGTTTATTTAAAAAACATATAATTGTTCTCTAGTGTAATG
TAATGGAACAGACGTGGATAATATTTATAATTTATAATTAGTCATTAAACATTATCATAT
GTTTAAAATTGGAAACATTCGTTGTACGTCAAATATTAGTTGTTATCTTACTGCATAAAC
AAAAAAAAAGACGAGAAATTGTAGTTTTAAACATTACTTAATTAGTTAAGCTTTAATATT
ACGTACAAATTTCACTTAACTTAAGATAAAATAAACAAAATTGAAACTGGTTTGGTGCTT
AAGCTTGAGCAAGAAGGTAATGCAAACGAAGCACACTACACAGAGATTCTGGATGAAGAA
CAAATCCACATGAAATTCGATTCTCACATTTCTTCTATTTAATTCGAATTTCAAATTGCC
ATTTCTCAGATTCCGGGGAAAGAAAAAAAAACCTAGAAAAGTGTTTTCTCCGTTTTCCAA
TCATCCATGAGTCCGGACAATAAACTGCTGGTCATGTAGATGGCTTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAA
   
Sequence Name 16-K117386-0022-333-B07C-8409
GenBank Accession LN493606 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K117386-0022-333-B07C-8409
GCATTTCATTGAGCCATCTACATGACCAGCAGTTTATTGTCCGGACTCATGGATGATTGG
AAAACGGAGAAAACACTTTTCTAGGTTTTTTTTTCTTTCCCCGGAATCTGAGAAATGGCA
ATTTGAAATTCGAATTAAATAGAAGAAATGTGAGAATCGAATTTCATGTGGATTTGTTCT
TCATCCAGAATCTCTGTGTAGTGTGCTTCGTTTGCATTACCTTCTTGCTCAAGCTTAAGC
ACCAAACCAGTTTCAATTTTGTTTATTTTATCTTAAGTTAAGTGAAATTTGTACGTAATA
TTAAAGCTTAACTAATTAAGTAATGTTTAAAACTACAATTTCTCGTCTTTTTTTTTGTTT
ATGCAGTAAGATAACAACTAATATTTGACGTACAACGAAGGTTTCCAATTTTAAACATAT
GATAATGTTTAATGACTAATTATAAATTATAAATATTATCCACGTCTGTTCCATTACATT
ACACTAGAGAACAATTATATGTTTTTTTAAATAAAACAGAGATTAAAAGGTCAATTTGGA
TTAGGTTTAAATTTTAACGTGGCAAACCACCA

Last Updated on 06.12.2018 16:11