Sequences from confirmation amplicon


Confirmation sequences for line 334B02 and hit At5g23160 are shown.
Line ID 334B02
Locus At5g23160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7796309
Primers used for PCR 19xc - 8474, show primer details
   
Sequence Name 43-K119175-0022-334-B02C-19xc
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 19xc: 5'-AGTATGGTTTGGAAAGTTAAACCG-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K119175-0022-334-B02C-19xc
TTTTCATGGACCGGTGAGACATATTAATTCATCCAATCTCGATCAACTTGTACTAGGCTA
TTAACACAAATACATGTTATCCAACTTGTAAACACAAATAGAATTACCACATATCGTTTG
GGTCGGGCATGTCTCCACCCGAACCCCGAGGCCCGAGGCCCCGAGCTTTGGACCTTCCGA
CTTGTTGTTGGTCGGGTAGGGAGATTCATGTGCTACCACAAACAACTTGTGAAAAGATTC
AAAATATATCAACAATGATCAAGATAATTTTCACGACCGTAATTATAACTTGGAGGTAGT
AGATACTATTTTTTTCATCTTATTCTTTTTATCGTATTTCCGAAAGCGTGCGAACACTAT
CACAAGTATTTCAGTTAATAAAAAATGGGATATAATTCCAACCGTTCCGAGTCATATATA
TAATGTCAAGTCTTCACACAAATTAATGTTACATTTTTGGATACTTCTTTTCATCTTATA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 15-K119174-0022-334-B02C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K119174-0022-334-B02C-8409
ATTATAAGATGAAAGAAGTATCCAAAAATGTAACATTAATTTGTGTGAAGACTTGACATT
ATATATATGACTCGGAACGGTTGGAATTATATCCCATTTTTTATTAACTGAAATACTTGT
GATAGTGTTCGCACGCTTTCGGAAATACGATAAAAAGAATAAGATGAAAAAAATAGTATC
TACTACCTCCAAGTTATAATTACGGTCGTGAAAATTATCTTGATCATTGTTGATATATTT
TGAATCTTTTCACAAGTTGTTTGTGGTAGCACATGAATCTCCCTACCCGACCAACAACAA
GTCGGAAGGTCCAAAGCTCGGGGCCTCGGGCCTCGGGGTTCGGGTGGAGACATGCCCGAC
CCAAACGATATGTGGTAATTCTATTTGTGTTTACAAGTTGGATAACATGTATTTGTGTTA
ATAGCCTAGTACAAGTTGATCGAGATTGGATGAATTAATATGTCGTCACCGGTCCATGAA
AATGATGCGAACGTAATTCGAAGCTAGCTACGGTTTAACTTTCCAAACCATAC

Last Updated on 06.12.2018 16:11