Sequences from confirmation amplicon


Confirmation sequences for line 334F09 and hit At4g20720 are shown.
Line ID 334F09
Locus At4g20720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11105496
Primers used for PCR 10vb - 8474, show primer details
   
Sequence Name 56-K117737-0022-334-F09C-10vb
GenBank Accession LN509222 [GenBank]
Sequencing primer gene specific primer, 10vb: 5'-AGCATCTTTCTCCTCAAAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K117737-0022-334-F09C-10vb
CTTTCCTGCGCATCTCGTCCAGCAAAAAACTAAGATTCTCATGTTCCCCAGACGATACAA
CACTCTTAGGTGCATCTTTTTTATCAAACAAACCAACATTATCATGCTGTTGCGATCCAA
CAACTCCCTGACTCTTTACACTATCGAATAAGCTAAGACTACGTGGATCCTTAAAATCAT
CATCCTCCACAGGTTTACTCTCTGCCTGCTCCACTTTGGAAAGATCTCCTCTCCGCTCAA
CGAAATAATCATCAAGATTAATCCCGCCTAGATTCAGAGGGTTTTTCTTCTGTACTGATT
GTCCATCATCAGACTTCTCCTCTAATGCAGACCATTGTATTTCCAAATCAAGAAACTTAG
ACAAAGCAATTCCTTTTGAAGGCGGTGCCTTAGTAGCTCCTCTGCTTGACCCATCCGTTT
CCTTTAGTGGCTCTACCATCTCCTACAGATGAAAAGTTCGTTTTTTTGAAGACACAACAC
ACCACATTCTACATCTGACTAATACACAATCAGCTAGGAAAACAAGTAATTACAAATTAG
TGAGAAAGGTATCGACTTTTACCGATCCATCGAGATTAAGAGAGGTGAGAAACCATTTGT
AAGCAGAAGTGGAAGTAAATTTGATGGGATCAGGAGGATTATCACTCGTGCGTTGCTGAT
TTCCACAGAAGACACAGATCAGAGACTCGATTCCTCGTAAAAATTTTCCTTTGCAGTTTC
GGCAACGAAGGTAGGGAGCTGAAGCATCAAGTTCTGCGATAGCCTCCTCGGATGTGGGAA
AGCTGGGGA
   
Sequence Name 24-K117736-0022-334-F09C-8409
GenBank Accession LN497167 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K117736-0022-334-F09C-8409
TCGGACTCATCCTTCCCCAGCCTTCCCACATCCGAGGAGGCTATCGCAGAACTTGATGCT
TCAGCTCCCTACCTTCGTTGCCGAAACTGCAAAGGAAAACTTTTACGAGGAATCGAGTCT
CTGATCTGTGTCTTCTGTGGAAATCAGCAACGCACGAGTGATAATCCTCCTGATCCCATC
AAATTTACTTCCACTTCTGCTTACAAATGGTTTCTCACCTCTCTTAATCTCGATGGATCG
GTAAAAGTCGATACCTTTCTCACTAATTTGTAATTACTTGTTTTCCTAGCTGATTGTGTA
TTAGTCAGATGTAGAATGTGGTGTGTTGTGTCTTCAAAAAAACGAACTTTTCATCTGTAG
GAGATGGTAGAGCCACTAAAGGAAACGGATGGGTCAAGCAGAGGAGCTACTAAGGCACCG
CCTTCAAAAGGAATTGCTTTGTCTAAGTTTCTTGATTTGGAAATACAATGGTCTGCATTA
GAGGAGAAGTCTGATGATGGACAATCAGTACAGAAGAAAAACCCTCTGAATCTAGGCGGG
ATTAATCTTGATGATTATTTCGTTGAGCGGAGAGGAGATCTTTCCAAAGTGGAGCAGGCA
GAGAGTAAACCTGTGGAGGATGATGATTTTAAGGATCCACGTAGTCTTAGCTTATTCGAT
AGTGTAAAGAGTCAGGGAGTTGTTGGATCGCAACAGCATGATAATGTTGGTTTGTTTGAT
AAAAAAGATGCACCTAAGAGTGTTGTTTCTTCTGGGGAACATGAGAATCTTAGTTTGTTT
GCTGGACGAGATGCGCAGGAAAGTGTTTCTTTCGCAGCGCAGGGAAACTTTGGGTTCTTT
GA

Last Updated on 06.12.2018 16:11