Sequences from confirmation amplicon


Confirmation sequences for line 334G08 and hit At4g21060 are shown.
Line ID 334G08
Locus At4g21060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11242363
Primers used for PCR 37qf - 8474, show primer details
   
Sequence Name 27-K115635-0022-334-G08C-37qf
GenBank Accession LN498533 [GenBank]
Sequencing primer gene specific primer, 37qf: 5'-TCAACCATAAATTGTGAAACCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K115635-0022-334-G08C-37qf
AACAGATTCCTTTATGAGCATACTGTGGAGTTCCAAGAATTGTAATAGAAGATCCTGCAG
CAAGACCACAGGGAAGCAACATGATCCTATTGGCTTTATTCAAGTCATCTCCATTCATTG
AGATCTGAGAAGGACATGACTCCACCTTTCCTTCGAATATAGAAGCACTCTCGTTGATCT
TATCAACCTCAAACTTATCAACATCTTCCCAAGCCTTGGATCCAAGTATCCAAGCCTCAT
CTGCCATTCTTTCAAAAGGTGACATATGAATAGTCCTATTCCTACGTCTCATGACCTCTC
CATATATCATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTC
   
Sequence Name 13-K115634-0022-334-G08C-8409
GenBank Accession LN492181 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K115634-0022-334-G08C-8409
GAGGTCATGAGACGTAGGAATAGGACTATTCATATGTCACCTTTTGAAAGAATGGCAGAT
GAGGCTTGGATACTTGGATCCAAGGCTTGGGAAGATGTTGATAAGTTTGAGGTTGATAAG
ATCAACGAGAGTGCTTCTATATTCGAAGGAAAGGTGGAGTCATGTCCTTCTCAGATCTCA
ATGAATGGAGATGACTTGAATAAAGCCAATAGGATCATGTTGCTTCCCTGTGGTCTTGCT
GCAGGATCTTCTATTACAATTCTTGGAACTCCACAGTATGCTCATAAAGAATCTGTTCCT
CAGCGTTCGAGATTGACAAGAAGCTATGGAATGGTTTTGGTTTC

Last Updated on 06.12.2018 16:11