Sequences from confirmation amplicon


Confirmation sequences for line 334H07 and hit At3g04010 are shown.
Line ID 334H07
Locus At3g04010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1039247
Primers used for PCR 59qf - 8474, show primer details
   
Sequence Name 36-K115400-0022-334-H07C-59qf
GenBank Accession LN502346 [GenBank]
Sequencing primer gene specific primer, 59qf: 5'-AGCTTACCTCAGTTTGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K115400-0022-334-H07C-59qf
ATCCACTATTATTATTGTTTTCACATTTTATTTTCTATCATTTTGTCATCATTTTTAATT
TTTTTGTTCATACAAAATAAAATAATAATACAAATTCAAAAATAATCAACTGAAAATAAT
GTTCTGTGAAATTCCAATATTCCAAAAGGGTCTAATATCTGATTTCCCCTTTTCAGATCA
CTACCCAATATCTAAAAACTTAAGTAGTCCTAAGAAACTTCTTACCTTATGTTAAATAAC
AAAACAATGAGTTTCAAACTTACTTGATCTTAACGCCGTTGTTAAAGTTGAATCTAGAGA
CGTTTCTTTGGACCCAATCTTTAGCTCTTTGATAGTTTCCCATCGCTAGGAGCAGATCAT
TAGGGATAGCCACCATCACTTCGACGCCGGAGCCGGCGAGAGCTCCCATGGTGTTTGTGT
CGGCATCGAACAACTTCACTTTCTGTATATTGTTGTCTTTAAGCATCTCCACCACCGTCT
TCGGCGGTAACTGATGACTCGCCATTGTTCCCCAGTTTACTCCCAAACCAGAGACACCGC
CGGAGAGAAAACATACGGCGGAGATGATGATAAAGACAACGGCAGAGTTAGTTATAGCAC
TCTGTTTACGGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 12-K115399-0022-334-H07C-8409
GenBank Accession LN491712 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K115399-0022-334-H07C-8409
CATTTCATTGAATATATCCTGCCGTAACAGAGTGCTATAACTAACTCTGCCGTTGTCTTT
ATCATCATCTCCGCCGTATGTTTTCTCTCCGGCGGTGTCTCTGGTTTGGGAGTAAACTGG
GGAACAATGGCGAGTCATCAGTTACCGCCGAAGACGGTGGTGGAGATGCTTAAAGACAAC
AATATACAGAAAGTGAAGTTGTTCGATGCCGACACAAACACCATGGGAGCTCTCGCCGGC
TCCGGCGTCGAAGTGATGGTGGCTATCCCTAATGATCTGCTCCTAGCGATGGGAAACTAT
CAAAGAGCTAAAGATTGGGTCCAAAGAAACGTCTCTAGATTCAACTTTAACAACGGCGTT
AAGATCAAGTAAGTTTGAAACTCATTGTTTTGTTATTTAACATAAGGTAAGAAGTTTCTT
AGGACTACTTAAGTTTTTAGATATTGGGTAGTGATCTGAAAAGGGGAAATCAGATATTAG
ACCCTTTTGGAATATTGGAATTTCACAGAACATTATTTTCAGTTGATTATTTTTGAATTT
GTATTATTATTTTATTTTGTATGAACAAAAAAATTAAAAATGATGACAAAATGATAGAAA
ATAAAATGTTGAAAACAATAATAATAGTTGGATTAGGAGGTCTATAATAGTCCTGAGCCC
ACTAGATTAGACAAACTGGGGTAAGCT

Last Updated on 06.12.2018 16:11