Sequences from confirmation amplicon


Confirmation sequences for line 335F03 and hit At2g39840 are shown.
Line ID 335F03
Locus At2g39840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16627811
Primers used for PCR 20kh - 8474, show primer details
   
Sequence Name 47-K113756-0022-335-F03C-20kh
GenBank Accession LN506256 [GenBank]
Sequencing primer gene specific primer, 20kh: 5'-TGCGCGTAATTTTCTTCTTTATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K113756-0022-335-F03C-20kh
TTGCTTACTCTTTCTTTTTTGGGGTCGAAAAAATTAGGTTTTTTTTTGGGATTCTGTTGA
TGGCGACGACGACGACGACGCAGGGGCAGCAAACGGCGATTGACTCGGCGGTTCTCGATG
ATATAATCAGGCGACTCACGGAGGTCCGGTTAGCTAGACCAGGAAAGCAAGTCCAGCTCT
CTGAAGCTGAGATCAAACAGCTCTGTACTACAGCTAGAGATATTTTCCTTCAGCAACCCA
ATTTACTTGAGCTTGAGGCTCCCATCAAAATCTGTGGTATCTTCCCAATTTGATTCGTTG
ATTTTAATAATTTCGTCGGAATTGATTTTAGGGTTCTTATTGTGTTTCTTAGATTTTCCA
AAAGTGTGAACTTGGTTAGGTTTTGGGGATAAAGTTGTTAGACCATTGTGAGACCTTTTT
GTTTGATGTTGTGAAGTGACTTCATGGCTTCATGTCCGGGAAATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 09-K113755-0022-335-F03C-8409
GenBank Accession LN490262 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K113755-0022-335-F03C-8409
AGCCATTTACATTGAATATTTCCCGGACATGAAGCCATGAAGTCACTTCACAACATCTAA
CAAAAAGGTCTCACAATGGTCTAACAACTTTATCCCCAAAACCTAACCAAGTTCACACTT
TTGGAAAATCTAAGAAACACAATAAGAACCCTAAAATCAATTCCGACGAAATTATTAAAA
TCAACGAATCAAATTGGGAAGATACCACAGATTTTGATGGGAGCCTCAAGCTCAAGTAAA
TTGGGTTGCTGAAGGAAAATATCTCTAGCTGTAGTACAGAGCTGTTTGATCTCAGCTTCA
GAGAGCTGGACTTGCTTTCCTGGTCTAGCTAACCGGACCTCCGTGAGTCGCCTGATTATA
TCATCGAGAACCGCCGAGTCAATCGCCGTTTGCTGCCCCTGCGTCGTCGTCGTCGTCGCC
ATCAACAGAATCCCAAAAAAAAACCTAATTTTTTCGACCCCAAAAAAGAAAAGAGCTAAT
GCAAAATAATCAGTGAGAACAAAATAAAGAAGAAATTTCCGCGC

Last Updated on 06.12.2018 16:11