Sequences from confirmation amplicon


Confirmation sequences for line 336B09 and hit At2g21540 are shown.
Line ID 336B09
Locus At2g21540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9223514
Primers used for PCR 13ym - 8474, show primer details
   
Sequence Name 31-K120479-0022-336-B09C-13ym
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 13ym: 5'-CACATGGTACTTGACATACCTATCG-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K120479-0022-336-B09C-13ym
CACTTGGCCAGCCTCTCAATGTAACAGGCCTTCCATCTTTGTCAACACCATGGTAACCTT
GAGGGTAGTACTTGAGGACTTCATCAATCTCCTTGAAATCAAAGTCCTTTTGAAAGAAGA
AGTAAAAAGGATCAGACTTTAATTAAAGGAGAAGAAAAAGAAGATGATCTTAAAATGTTT
AGGACCTCCATGATTGTGTCAACACCAAACTCTTTCCTCCAATGGATCATATCAGTCCAC
ATTTGCTTTGCTTTCTCAAGATCAAACTTCCTTGCCCTCAGAAACCTCAGCATCATGTGA
TGATCATCGTGTTTGGAAGGGAGAAGCTCGTCTAGGATAAGAGCTTGACGGAAAGCGTCA
ACAGCTTGAAGCTCTTCAAGATCAATATCGTCAACAATAGAGACAGACATGACTCGGCTG
TTCCTTCGAGTTCTCTTCGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 07-K120478-0022-336-B09C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K120478-0022-336-B09C-8409
CATTTCATTGAATTATCCTGACGAAGAGAACTCGAAGGAACAGCCGAGTCATGTCTGTCT
CTATTGTTGACGATATTGATCTTGAAGAGCTTCAAGCTGTTGACGCTTTCCGTCAAGCTC
TTATCCTAGACGAGCTTCTCCCTTCCAAACACGATGATCATCACATGATGCTGAGGTTTC
TGAGGGCAAGGAAGTTTGATCTTGAGAAAGCAAAGCAAATGTGGACTGATATGATCCATT
GGAGGAAAGAGTTTGGTGTTGACACAATCATGGAGGTCCTAAACATTTTAAGATCATCTT
CTTTTTCTTCTCCTTTAATTAAAGTCTGATCCTTTTTACTTCTTCTTTCAAAAGGACTTT
GATTTCAAGGAGATTGATGAAGTCCTCAAGTACTACCCTCAAGGTTACCATGGTGTTGAC
AAAGATGGAAGGCCTGTTTACATTGAGAGGCTAGGCCAAGTTGATGCCACAAAGCTGATG
CAAGTCACTACCATCGATAGGTATGTCAGTAACCATGTG

Last Updated on 06.12.2018 16:11