Sequences from confirmation amplicon


Confirmation sequences for line 336D12 and hit At2g19800 are shown.
Line ID 336D12
Locus At2g19800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8532353
Primers used for PCR 40qf - 8474, show primer details
   
Sequence Name 48-K118051-0022-336-D12C-40qf
GenBank Accession LN506634 [GenBank]
Sequencing primer gene specific primer, 40qf: 5'-AGAGACAACAAGGTGTTGAGGAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K118051-0022-336-D12C-40qf
ATGACTTTGTAAGCAAACAAACATACTTTCACTATGGTCTAGTTAATTAAATTATAAGTA
AATAATGTTTATTGAAAATCGATCTTCAAACAATTGGACAGGTGAAGAAGATGAGGAAAG
AGTATGGAAAACTTAACAAGATGGAAATGAGTATATGGGAATGTTGTGAGTTATTGAACA
ATGTTGTTGATGAAAGCGATCCGGATCTTGATGAGCCTCAAATTCAACACCTTCTCCAAA
CCGCTGAAGCCATTCGAAGGGACTATCCCGACGAAGATTGGCTCCATCTCACTGCCCTAA
TCCATGGTACATGTCTTATTCTGCTATATCATAAAAAATATACACCACTTTTGAAAAATC
TACCATTTATAGTGTTCAACAAAGCATCCTGACTATGTATTCTTTAGTCTTTTTTTCTTC
TTATGGTTTATATTGAGAAATTTCAAAATGATAAGAGCAAACGATTTGTTATGTTGAACA
ATTTTGAAAGTTCATATAGCTTCTTATACTGGTTATGTTACTATTCAACATATTAAATGC
ATATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 12-K118050-0022-336-D12C-8409
GenBank Accession LN491781 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K118050-0022-336-D12C-8409
GAAAGTAACATAACCAGTATAAGAAAGCTATATGAACTTTCAAAATTGTTCAACATAACA
AATCGTTTGCTCTTATCATTTTGAAATTTCTCAATATAAACCATAAGAAGAAAAAAAGAC
TAAAGAATACATAGTCAGGATGCTTTGTTGAACACTATAAATGGTAGATTTTTCAAAAGT
GGTGTATATTTTTTATGATATAGCAGAATAAGACATGTACCATGGATTAGGGCAGTGAGA
TGGAGCCAATCTTCGTCGGGATAGTCCCTTCGAATGGCTTCAGCGGTTTGGAGAAGGTGT
TGAATTTGAGGCTCATCAAGATCCGGATCGCTTTCATCAACAACATTGTTCAATAACTCA
CAACATTCCCATATACTCATTTCCATCTTGTTAAGTTTTCCATACTCTTTCCTCATCTTC
TTCACCTGTCCAATTGTTTGAAGATCGATTTTCAATAAACATTATTTACTTATAATTTAA
TTAACTAGACCATAGTGAAAGTATGTTTGTTTTGCTTACAAAGTCATAGGTCTGGTGAAT
GTGTTGCATCCTGTAAAATTCCTCAACACCTGGTTTTGTCTC

Last Updated on 06.12.2018 16:11