Sequences from confirmation amplicon


Confirmation sequences for line 336D12 and hit At2g32600 are shown.
Line ID 336D12
Locus At2g32600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13833753
Primers used for PCR PF03 - 8474, show primer details
   
Sequence Name 49-K118152-0022-336-D12C-PF03
GenBank Accession LN506979 [GenBank]
Sequencing primer gene specific primer, PF03: 5'-GTGGATCCGAGAAAATTCAAAATA-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K118152-0022-336-D12C-PF03
TTTGATTTTCCCTCTTCTTACCCTTTTGAATTTCGAGACACTGATGAGAATGTGTAAATA
CATGATTACCCCCGTCGAAAGGTGAGTGACGTGGTATACTCCACTTTGATCATCGTTAGA
AATAATTTAAATATGAAATATCCATGGCCGGTCATAGGACTGATAGATTGGGTTAAGACT
TGGTTAAGAGTTAACCGATATATAACAAATGAATTTTGTTTCGCCAACGCTAATTGTACT
ATTAACGGAAAACAATCTTGTCAAAATGGTACATACGAGAAGCAGATAGAAGAAACTCAA
AGAAGGTATCTAGAGAGCTAATACATCCGTAGAGATCACAGCTTTGAGAATCCATGTCAA
AACAGATTTGTTTTTCAGTCAAAGAGCAAGTCTCTCTTAGTAAACCATGATCAGAGTACT
GTGTTTTGGATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATATCCGCACGGTTATT
   
Sequence Name 01-K118151-0022-336-D12C-8409
GenBank Accession LN486772 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K118151-0022-336-D12C-8409
TCGGATGAGCATTTACATTGAATATCCAAACACAGTACTCTGATCATGGTTTACTAAGAG
AGACTTGCTCTTTGACTGAAAAACAAATCTGTTTTGACATGGATTCTCAAAGCTGTGATC
TCTACGGATGTATTAGCTCTCTAGATACCTTCTTTGAGTTTCTTCTATCTGCTTCTCGTA
TGTACCATTTTGACAAGATTGTTTTCCGTTAATAGTACAATTAGCGTTGGCGAAACAAAA
TTCATTTGTTATATATCGGTTAACTCTTAACCAAGTCTTAACCCAATCTATCAGTCCTAT
GACCGGCCATGGATATTTCATATTTAAATTATTTCTAACGATGATCAAAGTGGAGTATAC
CACGTCACTCACCTTTCGACGGGGGTAATCATGTATTTACACATTCTCATCAGTGTCTCT
GAAATTCAAAAGGGTAAAGAAGAGGGAAAATCAAAATATTAAAATCCCTAATTCCCAAAT
TATTTTGAATTTTCCGGGATCCAC

Last Updated on 06.12.2018 16:11