Sequences from confirmation amplicon


Confirmation sequences for line 336E01 and hit At4g29800 are shown.
Line ID 336E01
Locus At4g29800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14591214
Primers used for PCR 25ay - 8474, show primer details
   
Sequence Name 69-K106975-0022-336-E01C-8409
GenBank Accession LN512214 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K106975-0022-336-E01C-8409
GAGCCTTTACATTGAATAAAATCATTAGAGAAATAAATTGTGGTGCAGCAGGCAAATGGA
TCGCGATTAGGAGCTTGTGGACCGAACGTGGACACGGATCCAAGAGCAGAGAATGTGAAG
AAACTGACGGAGATAGCTGATGAGATGTTGAAGCAAAACAATGTGGAGTCGGTTTTGTTC
GGAAGCAAAAGGATAGGAGAAATGAGCAATTCAGAGAAAATAGAGTGGTTTGCGAGCGAG
CTTGTCATTGAGCAGCAGCGACGAAGTGTTAGAGCGTCTCCAACCGTAACGCTAAAGCAA
GCTGTGTCCAAAACTAACCGCAATGCTATAAACGCAACTCTCACTCTCATATCTAAAGAT
AGATGAAAACACCTGTTTTTGGTGCTTCCTATTGGTTTTTCATACCTTTTGTTTCGACGT
TGATATTTACTTGAAAGGGAGAATTAGAGTGCGATTCAAATATACAAGTCATTATTTTAG
TTTCATATTTCATAATGTCCTTTCAAAATTCATTTTGATATCTTAACTCCAAGTTATTCA
ATTCATAACACTATATGTTAGCAAATCGAAGGCGTGATTGATGATGACTTGTTAGCATAA
TATGTTAGAGTGAGACCTGNNTTCTTGCA
   
Sequence Name 85-K106977-0022-336-E01C-25ay
GenBank Accession LN514549 [GenBank]
Sequencing primer gene specific primer, 25ay: 5'-TGCAAGAAACAGGTCTCACTCTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K106977-0022-336-E01C-25ay
TCATCATCACGCCTTCGATTTGCTAACATATAGTGTTATGAATTGAATAACTTGGAGTTA
AGATATCAAAATGAATTTTGAAAGGACATTATGAAATATGAAACTAAAATAATGACTTGT
ATATTTGAATCGCACTCTAATTCTCCCTTTCAAGTAAATATCAACGTCGAAACAAAAGGT
ATGAAAAACCAATAGGAAGCACCAAAAACAGGTGTTTTCATCTATCTTTAGATATGAGAG
TGAGAGTTGCGTTTATAGCATTGCGGTTAGTTTTGGACACAGCTTGCTTTAGCGTTACGG
TTGGAGACGCTCTAACACTTCGTCGCTGCTGCTCAATGACAAGCTCGCTCGCAAACCACT
CTATTTTCTCTGAATTGCTCATTTCTCCTATCCTTTTGCTTCCGAACAAAACCGACTCCA
CATTGTTTTGCTTCAACATCTCATCAGCTATCTCCGTCAGTTTCTTCACATTCTCTGCTC
TTGGATCCGTGTCCACGTTCGGTCCACAAGCTCCTAATCGCGATCCATTTGCCTGCTGCA
CCACAATTTATTTCTCTAATGATTTTATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGAT

Last Updated on 06.12.2018 16:11