Sequences from confirmation amplicon


Confirmation sequences for line 337A01 and hit At5g12150 are shown.
Line ID 337A01
Locus At5g12150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3925503
Primers used for PCR RU36 - 8474, show primer details
   
Sequence Name 06-K023834-022-337-A01C-8409
GenBank Accession LN488630 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K023834-022-337-A01C-8409
ATCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAAATGAAAT
GACAATCTATCCTCTATTTTTTTTGGGGTATAATTTTGAGGGGAAATAAACTTCCATTTT
GCTGACCAAGCTTGCCATGAAATTGTCAGGTGGGTAGATTACAAGAGCAACTGCAAGCTG
AGAGAGACCTACGATCTGCTCTTGAAGTTGGTCTAAGCATTTCTTGTGGACAATTTAGCT
CACAAGCTGCGGATTCCAAAGTCAGTATACACTTTAGCTAGAGAAAGCAGTTTTTTTTTT
TTGGTTCTATCTCTGGCTTTCCTTAGTTCTAACATCCCAATATAATGCAAAGACAAGGGC
AAAGCTTGAGGAGATTGCTCTTGCTGAACCTGATGTCCCAAGGTTGAAGCAAAAAGTCCC
CAAATTACATCATCAGCTAAGTCAGCAGCGACAGCATCACTTAAGTTCACTCCCAGATGC
TCAAAGCCATCACCAGTTTCTCCATAATCACAATACTCAACTGTGAGTTTACACTCGATG
CCGGGTTCTAGCTCTTATTCAATAGTTCAAACGAGATATTACTCGAATGTAA
   
Sequence Name 10-K023834-022-337-A01C-RU36
GenBank Accession LN490475 [GenBank]
Sequencing primer gene specific primer, RU36: 5'-GTACCGTTCTCTGATTTCTTTCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K023834-022-337-A01C-RU36
AACGAAAGCAAGAATGGAATCAAAGTCCTGTTGAAAAGACTTCCTGCGAATTTGAAAAAA
AATAGATTGTTATTTCTCTTAGCGAGTTTACATTCGAGTAATATCTCGTTTGAACTATTG
AATAAGAGCTAGAACCAGGCATCGAGTGTAAACTCACAGTTGAGTATTGTGATTATGGAG
AAACTGGTGATGGCTTTGAGCATCTGGGAGTGAACTTAAGTGATGCTGTCGCTGCTGACT
TAGCTGATGATGTAATTCGGCGACTTTCTGCTTCAACCTTGCGACATCAGCTTCAGCAAG
AGCAATCTCCTCAAGCTCTGCCCTTGTCTTTGCATTATATTGTGATGTTAGAACTAAGGA
AAGCAAGAGATAGAACCAAAAAAAAAAAACTGCTTTCTCTAGCTAAAGTGTATACTGACT
TTGGAATCCGCAGCTTGTGAGCTAAATTGTCCACAAGAAATGCTTAGACCAACTTCAAGA
GCAAATCGTAGGTCTCTCTCAGCTTGCAGTTGCTCTTGTAATCTACCCACCTGACAATTT
CATGGCAAGCTTGGTCAGCAAAA

Last Updated on 06.12.2018 16:11