Sequences from confirmation amplicon


Confirmation sequences for line 337E11 and hit At4g30980 are shown.
Line ID 337E11
Locus At4g30980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15079528
Primers used for PCR 18uv - 8474, show primer details
   
Sequence Name 35-K117403-0022-337-E11C-18uv
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 18uv: 5'-CGAGTTTTAAACAAACTCTTTGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K117403-0022-337-E11C-18uv
AAAATTTAAGGGAATAACTTTATAAACCAAAAAAAAAAAACCTGGATACCTGGGGGGGCC
TTAGGGGGGGTTTGAATTGGGAAAAACCAAAAACCCAAGGGGTCCCCAAATCCGGGGCCT
TACCCAAAACCCCACCCTTTTTTTTTCAAACTTTTGCCCAAACCCCCCTTCCCCCTTTGC
CTTGGCCTCCAAACCTTTTTCCCCCCTTCCCCCCCCAAAAAGGGAAAAATAAATGGAACA
ATGGCCCTTTTCCCCGGTTGGAAACCGGAGGGACCTTTTTTACGGGGACCGGGAAATTCT
CCTGGGCTCCAAACCAAGGGATCCAACCAAACCCAAGGGACCATCCTCCTTGTAAAGGGC
TCCTTGCCCGGAAATTCTCCTGGGATCCCCATGGATTTTAAGGGCCAATTGGGAAAAAAA
AAAAAATTATTTCCCATTTTTTTTCCATTTCAAAA
   
Sequence Name 11-K117402-0022-337-E11C-8409
GenBank Accession LN491302 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K117402-0022-337-E11C-8409
ATCCTTTGTCTCGTAGCCATGTAGATTTCCCGGTCACCGTTAAAGACGCTCCCTCCGTTT
CCAAGCCGTGATAACGGCCATTTGTCCATTTCATTTTCCCTTTTTTGGGTGGGAAAGAGA
GAAAAAAGTTTAGAAGACAAAGACAAGTGGGATAGGTGGTTTTGGTCAAAGTTTAGAAAG
AATAAGGTCGTGTTTTCGGATACGACACCGTATTTGCGTACACTTTGGTTTTCTGTCTTT
ACCTACTACAAACCACCCATAAGCACACTCATGTTATCATGTTTTTTTTTTTTGGGTTAA
AAAAGTTAAACCCTAAATTTTTCAACCAAAAAACCGGTTGGGTCAAAAAAAGGTCGCCAA
GGATAATCTTTTGGGCAAAAAATTTTTTTAAAAACCGA

Last Updated on 06.12.2018 16:11