Sequences from confirmation amplicon


Confirmation sequences for line 337G08 and hit At1g43620 are shown.
Line ID 337G08
Locus At1g43620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:16426042
Primers used for PCR 09kt - 8474, show primer details
   
Sequence Name 39-K113817-0022-337-G08C-09kt
GenBank Accession LN503422 [GenBank]
Sequencing primer gene specific primer, 09kt: 5'-TGAAATGGTTTTCTTCTTGCTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K113817-0022-337-G08C-09kt
TTTCACTACATTTTGCAGTTGCTACTGAAGTTCCTGAAAATGTATTCTTAGTGGAGGACT
GTCCTCACGATTGGTTGTTTCCTCAATGTTCAGCTGTGGTAAGCCCTCTTTAGATTTTCA
ATATATTACTACATTCACATCTCCGAAGAGTTATCTTCTAATTGAGGCTGTTTCTTGTGT
TGTGGATATAAAGGTTCATCATGGCGGTGCTGGAACCACAGCGACTGGACTAAAAGCTGG
GGTATAACATTTAAGTTGCTTTCTTTCATACTCATCACCGAAGTGTCCTGTGTTGGAATC
TTTAACAGTTTTATTGACCATATGAAACCTGTTACTTCCTATATTCTTATGAATTCCATT
TGTGTCATCAGTGTCCAACAACGATCGTGCCATTCTTTGGGGATCAGTTCTTCTGGGGTG
ACAGGATCTATGAGAAAGGACTTGGGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCA
   
Sequence Name 13-K113816-0022-337-G08C-8409
GenBank Accession LN492121 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K113816-0022-337-G08C-8409
ATCCTGTCCCCCAGAAGAACTGATCCCCAAAGAATGGCACGATCGTTGTTGGACACTGAT
GACACAAATGGAATTCATAAGAATATAGGAAGTAACAGGTTTCATATGGTCAATAAAACT
GTTAAAGATTCCAACACAGGACACTTCGGTGATGAGTATGAAAGAAAGCAACTTAAATGT
TATACCCCAGCTTTTAGTCCAGTCGCTGTGGTTCCAGCACCGCCATGATGAACCTTTATA
TCCACAACACAAGAAACAGCCTCAATTAGAAGATAACTCTTCGGAGATGTGAATGTAGTA
ATATATTGAAAATCTAAAGAGGGCTTACCACAGCTGAACATTGAGGAAACAACCAATCGT
GAGGACAGTCCTCCACTAAGAATACATTTTCAGGAACTTCAGTAGCAACTGCAAAATGTT
AGTTGGTAAAAATGTGTCAGGAATCAGTATAAAAGCAAGAAGAAACCCATTTC
Primers used for PCR 39vq - 8474, show primer details
   
Sequence Name 74-K117932-0022-337-G08C-39vq
GenBank Accession LN513143 [GenBank]
Sequencing primer gene specific primer, 39vq: 5'-ATCCTTCAAAGTCATTCTCATTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K117932-0022-337-G08C-39vq
GACAACCATAACAATAATTTTATTACATCCATTTTATGCAACTGTGCAAAAGAAATCTAA
TTATATTCCTGACCATTGAATGAAATGAATTGAGTTGCAGAGACATAGATTATGCAACTT
AAACACACGACTAAGGAACTTTGATATCACACGCCACCACATGGAAGACAACACTTCTTG
CCAATCTGAATAAAGAACCACTGTAACAGGTCTGGTCGATCATCTTCATCTTTTTTCTCG
GACGAGGACTCGGGCAGTGGCAGCTCTGGTGGCAAATGCCTGTGGAATGCATCAACTGCT
GCAGCTACACCATCCTCGTTCTCCAGTACTTTCGCTAGCTCCATCACTTGTGATTTCACC
TGAAAGCACATGATTCGTGTCCTATGATGTCTATGCTTTCTAGAGAAAGCTTTGGAGGTT
TTATGGAGGAGGCTTAACATCCTCCCTAGGCTTGGAAGATATTAAAAAAAAGGGAAAAGC
AAGTTTGTGTACCTCTGGTTGTAGCATGAATCTTATGGAGCTACTGAGGTTCTCAGGATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTC
   
Sequence Name 34-K117931-0022-337-G08C-8409
GenBank Accession LN501583 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K117931-0022-337-G08C-8409
GCCATTTCAATTGAATATATCCTGAGAACCTCAGTAGCTCCATAAGATTCATGCTACAAC
CAGAGGTACACAAACGTTGCTTTTCCCTTTTTTTAATATCTTCCAAGCCTAGGGAGGATG
TTAAGCCTCCTCCATAAAACCTCCAAAGCTTTCTCTAGAAAGCATAGACTTCAGAGGACA
CGAATCATGTGCTTTCAGGTGAAATCACGAGTGATGGAGCTAGCGAAAGTACTGGAGAAC
GAGGATGGTGTAGCTGCATCAGTTGATGCATTCCACAGGAATTTGCCACCAGAGCTGCCA
CTGCCCGAGTCCTCGTCCGAGAAAAAAGATGAATATGATCGACCAGACCTGTTACGATGG
TTCTTTATTCAGATTCGGCTAGAAGTGTTGTCTTCCATGTGGTGGCGTGTGATATCAAAG
TGTCCGAAGTCTTGTGTTTAACTTGCGTAAGCAATATCTCTGCAACTCGGTTCAATTCAG
TCAGTGGTCAGGAAGATGATTAGATTTCTTTTGCGCACTTCCGTGGTACGGAGGTAATAT
AATTATGGTTATGGGTTTGTGCATCGTAATTAGTTTGTGGTCCACTGAGAATGACATTGG
AAAGATAGGAGAAAAAGAANGAGTATTTACCAATTTTTTTTCAATTCAGAAATGTAGATC
TCCGCTCAAAAAA

Last Updated on 06.12.2018 16:11