Sequences from confirmation amplicon


Confirmation sequences for line 338B11 and hit At4g20800 are shown.
Line ID 338B11
Locus At4g20800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11140691
Primers used for PCR BV45 - 8474, show primer details
   
Sequence Name 92-K105954-0022-338-B11C-BV45
GenBank Accession LN515196 [GenBank]
Sequencing primer gene specific primer, BV45: 5'-CTACCTTGGGACGTGATCTATTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>92-K105954-0022-338-B11C-BV45
GGGCGCTACTTTGGCGTTTCCTCTCGTGGAAATCAACCTCGTCGATGTTCCAAAGACCGT
GACTGTTTTTAAGGTTAACAAAACCTTGGAGCAAGGAGTTACTGATGTTCTCTACAAATG
GCAGCTTGTGTCGAGCAAATTGCCTCAAGACCTTTTCTTGAGAGCAATGCCTAAACCGGT
AAATGGCGTGGTACCTAGCGAGAAAACCATCGCAGTTGTGTTCTACGCTCAGTTTTTAGG
CTCAGCAAGGAGGCTCATGGCGATTATGAACAAGAATTTGCCTGAATTAGGGCTAAAACG
TGAAGATTGCTACGAAATGAGCTGGATTAATACGACAACGTTTTGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 28-K105955-0022-338-B11C-8409
GenBank Accession LN498852 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K105955-0022-338-B11C-8409
CGGAATGAGCCAAAACGTTGTCGTATTAATCCAGCTCATTTCGTAGCAATCTTCACGTTT
TAGCCCTAATTCAGGCAAATTCTTGTTCATAATCGCCATGAGCCTCCTTGCTGAGCCTAA
AAACTGAGCGTAGAACACAACTGCGATGGTTTTCTCGCTAGGTACCACGCCATTTACCGG
TTTAGGCATTGCTCTCAAGAAAAGGTCTTGAGGCAATTTGCTCGACACAAGCTGCCATTT
GTAGAGAACATCAGTAACTCCTTGCTCCAAGGTTTTGTTAACCTTAAAAACAGTCACGGT
CTTTGGAACATCGACGAGGTTGATTTTCCACGAGAGGATAACGCCAAAGCTAGCGCCCCC
ACCGCCTCGAATAGCCCACAATAGATCACGTCCCAAGGTA

Last Updated on 06.12.2018 16:11