Sequences from confirmation amplicon


Confirmation sequences for line 338C03 and hit At5g01820 are shown.
Line ID 338C03
Locus At5g01820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:313431
Primers used for PCR JK06 - 8474, show primer details
   
Sequence Name 43-K021738-022-338-C03C-8409
GenBank Accession LN504694 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K021738-022-338-C03C-8409
CATTGATCATCACATTCATATTATTCGTATGTACTTGAGTTGGTTCTGGGTCCGTTTGGT
CTGATTCGTGCACAAGCTCGACGAAGAAAGGCCTGAGTGTATCTGTCCATAAATCTCGTC
CAGATGCAGCGGCTCTTTGTCTCTTCCTCACCTCTATCATAACCAGCTCGTCCGTTAGCT
GGTTTATCTCAACCACCATTGCGAAGTTACCTTTTTGCCCCTCGATCTTCATCCCCCATG
TTTCCTTCTTCGCCACCGTCAGATTCTCCGCCGACACGATCTCCTCCAGCCTCTCCACCA
CCCTCTCCGCCGTCCACGCCGACACGAACCGTTCCACTCGGTCGTACTTTCTGGCGTCTC
CGAAAAGACCGGAGAGATTGAACCCTGGAGATCCTGAGATGATGTCGAATGCGTTCATCC
TCCTTGCTCCCATCTCGCTGTCGGTCTCGTCCGCCGGGAGTTTCATATCGGAGTCCTCTA
GATGGAATTTAGACATCCGATCGTCGTAGCCCTGTTTGAACCATGGATCGTGGATGATCT
CTTCGATAGTGATCCTCGTCTGCGGATTCGTGTCTAAAAGCCGAGTCAGCAGTCGCCGGA
GATCCGGTGAGGTCCACTTGGGAATGCGAAATTCTCCCTTGTAGATTT
   
Sequence Name 44-K021738-022-338-C03C-JK06
GenBank Accession LN505042 [GenBank]
Sequencing primer gene specific primer, JK06: 5'-TATGTACCGGAAAATCTACAAGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K021738-022-338-C03C-jk6
TCACCGGATCTCCGGCGACTGCTGACTCNGGCTTTTAGACACGAATCCGCAGACGAGGAT
CACTATCGAAGAGATCATCCACGATCCATGGTTCAAACAGGGCTACGACGATCGGATGTC
TAAATTCCATCTAGAGGACTCCGATATGAAACTCCCGGCGGACGAGACCGACAGCGAGAT
GGGAGCAAGGAGGATGAACGCATTCGACATCATCTCAGGATCTCCAGGGTTCAATCTCTC
CGGTCTTTTCGGAGACGCCAGAAAGTACGACCGAGTGGAACGGTTCGTGTCGGCGTGGAC
GGCGGAGAGGGTGGTGGAGAGGCTGGAGGAGATCGTGTCGGCGGAGAATCTGACGGTGGC
GAAGAAGGAAACATGGGGGATGAAGATCGAGGGGCAAAAAGGTAACTTCGCAATGGTGGT
TGAGATAAACCAGCTAACGGACGAGCTGGTTATGATAGAGGTGAGGAAGAGACAAAGAGC
CGCTGCATCTGGACGAGATTTATGGACAGATACACTCAGGCCTTTCTTCGTCGAGCTTGT
GCACGAATCAGACCAAACGGACCCAGAACCAACTCAAGTACATACGAATAATATGAATGT
GATTGATCAATTGTAATATGGAGATCCAAATAAAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGT
TATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAG
GCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATNTCGACGTGT

Last Updated on 06.12.2018 16:11