Sequences from confirmation amplicon


Confirmation sequences for line 338E08 and hit At3g32047 are shown.
Line ID 338E08
Locus At3g32047 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:13062445
Primers used for PCR 15qp - 8474, show primer details
   
Sequence Name 54-K115558-0022-338-E08C-15qp
GenBank Accession LN508581 [GenBank]
Sequencing primer gene specific primer, 15qp: 5'-CTGTGTGGATTCATTGAGAACCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K115558-0022-338-E08C-15qp
TTTTTCTGACTCATGAGCTTATCCTTGGAGGCACTGACACCTCAGCGCAGACAATTGAGT
GGACAATGGCAGAAATCATTAATAAGCCTAACATTCTTGAGAAATTGAGAAAAGAACTCG
ATTCTGTTGTAGGAAAAACAAGGCTGATTGAAGAGAAGGATCTACCAAACCTCCCTTATC
TGCAATCGGTCGTCAAGGAAGGGCTAAGATTGCACCCACCAGCGCCTGTGTTTGGAAGGA
AAGTCCTAGAAGGATGTACGATTAAAGGCTATTATGTACCAAAGAACACAGCTCTTGTTG
TTAATGCTTATGCTGTGATGAGAGATCCCCATTATTGGGAAGAGTCCCACCAGGATATAT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGC
   
Sequence Name 51-K115557-0022-338-E08C-8409
GenBank Accession LN507599 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K115557-0022-338-E08C-8409
ATTTCATTGAATATATCCTGGTGGGACTCTTCCCATAATGGGGATCTCTCATCACAGCAT
AAGCATTAACAACAAGAGCTGTGTTCTTTGGTACATAATAGCCTTTAATCGTACATCCTT
CTAGGACTTTCCTTCCAAACACAGGCGCTGGTGGGTGCAATCTTAGCCCTTCCTTGACGA
CCGATTGCAGATAAGGGAGGTTTGGTAGATCCTTCTCTTCAATCAGCCTTGTTTTTCCTA
CAACAGAATCGAGTTCTTTTCTCAATTTCTCAAGAATGTTAGGCTTATTAATGATTTCTG
CCATTGTCCACTCAATTGTCTGCGCTGAGGTGTCAGTGCCTCCAAGGATAAGCTCATGTA
GTCAGAAAAATCAAGAACAATTAATTTTGTTGTCTTAGGTTCTCAATGATACCACACA

Last Updated on 06.12.2018 16:11