Sequences from confirmation amplicon


Confirmation sequences for line 338E08 and hit At5g64600 are shown.
Line ID 338E08
Locus At5g64600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25826369
Primers used for PCR 22ew - 8474, show primer details
   
Sequence Name 31-K110985-0022-338-E08C-22ew
GenBank Accession LN500213 [GenBank]
Sequencing primer gene specific primer, 22ew: 5'-TGAATGCAACTTTAGTCATTCCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K110985-0022-338-E08C-22ew
CAGATCAGGTATTTTGAACTTCAACAAACAAAAAATGCAGCTTACTTAATTAGTGGTTAT
GAGATTTATACTATTTCTAACTTACATTTTCGCTATGTCTCAGTGTTTTTTCAGATATTT
TTGACGAGGAACAATTCATTAAATCATTGCGAAGAGATGTCAAGGTTATTAAAAAGCTCC
CAAAGGAAGTGGAATCTCTACCTAGAGCAAGGAAGCATTTCACTTCTTGGTCTAGTGTTG
GATATTATGAAGAAATGACTCACTTGTGGAAGGAGTACAAGGTCGGTCTTAAGCAACTTC
TTTTACATTTTGCATTTGCTGTTTCATTCAACTGCTGATAGAATAATAACAATGCAGGTC
ATCCATGTCGCAAAATCAGATTCTCGCCTTGCAAATAATGACCCAATTGAATCAGGATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
ATCCGCACGNTTATT
   
Sequence Name 15-K110984-0022-338-E08C-8409
GenBank Accession LN493026 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K110984-0022-338-E08C-8409
TTTCATTGAATATATCCTGATTCATTGGGTCATTATTTGCAAGGCGAGAATCTGATTTTG
CGACATGGATGACCTGCATTGTTATTATTCTATCAGCAGTTGAATGAAACAGCAAATGCA
AAATGTAAAAGAAGTTGCTTAAGACCGACCTTGTACTCCTTCCACAAGTGAGTCATTTCT
TCATAATATCCAACACTAGACCAAGAAGTGAAATGCTTCCTTGCTCTAGGTAGAGATTCC
ACTTCCTTTGGGAGCTTTTTAATAACCTTGACATCTCTTCGCAATGATTTAATGAATTGT
TCCTCGTCAAAAATATCTGAAAAAACACTGAGACATAGCGAAAATGTAAGTTAGAAATAG
TATAAATCTCATAACCACTAATTAAGTAAGCTGCATTTTTTGTTTGTTTGAAGTTTCAAA
ATACCTTGAATCTTGCCAAAACGATCGCTTATCCAGCTCAGGAATGACAAGNTGCATTC

Last Updated on 06.12.2018 16:11