Sequences from confirmation amplicon


Confirmation sequences for line 339A06 and hit At5g60000 are shown.
Line ID 339A06
Locus At5g60000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24159678
Primers used for PCR 18yh - 8474, show primer details
   
Sequence Name 38-K122428-0022-339-A06C-18yh
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 18yh: 5'-TTCTGATTGATAAAAACGTGTGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K122428-0022-339-A06C-18yh
GTTATTGGTTAGATTTGAGCTCTTTAAAATGTGAAGGGTTATCAAATTTATCCGATCTGA
TTATATATATCCTACTGGTTTGTATATATATACTTATGTCTTAGCACTTAAAACTTTAAA
ACTGTGAAGGGTTATCAAATTTAATTAACTTGTTAGATCCACAATTTGGTAACTCATATG
ATTGCTACATCAAATTAAATTTATGTTTTTTTTTGTAAATTAAATATCCTTTGACCTTTA
TCATAATTTTTCTGTTTTAAAATAAATATAGTTAAATTTTTTAATTTTTTGGACATAAAT
ACCTAAAGCAGAGGATCGACTTGCAAAATCTTCTCTATTTCAAGGTTCCAAATTTATGTA
TCATGTCTATATTCCACATTTGATGCAGAATATTGTAACAGTTTAGTACTTGATTAATTA
ATATATTAGATGTTTCAAAACTAAACTAAAATTCAAGTCAACATTTAGATCAGAATATCA
AAATTCAATTTAAATTTTAGAGATATAATAAAAGTTTATATACATATGTTATATAAATTT
GTGTGAAAATGTCAAACAACACTATTTATAATGAATATAGTTTTGGTTTTTCCATTTGAT
ATGACAAGTTGAATTTGCGTTAACGATGCATGTAACAGAATTTGATAACATCCACTAGCT
TTATATCAAAAAAATTTTATATGTCTTTATATCAATATATATAGAGTTTGGCAAGTTCAC
ACTAAACTTGTGTGAACATTGAAAAAGCCAAACATTATTGGAGCAGTGTACATAATGCTA
GCATCTTATAATATCTTTTTAATTTTGCATAGTTGTTATTTGTTCAACAAAAAAATTGGT
AATAAAAATATAAATGATTTTTTTTTTGAAATTACTTTATTCATTT
   
Sequence Name 14-K122427-0022-339-A06C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K122427-0022-339-A06C-8409
TCGGACTGACCATTTCATTGAATATATCCTGTCTACATTGTAAATATATACTCATTTACA
CTCTTTTTCCGTGGTGATGTTCCTGTCTTCGTCACGTGCCACCGAACCCCTTGGCTCTCC
AAACTCGTACCTTTAGGTCAGAAGTTGCTGATAGAGATCCCAACCACTACCGGCGACAAT
GCTGTTGATGAGAAGGCGCAAGGATCTAGACCGTGGCAGCCAAGAACTTGCATAGGAACG
TTTTACAGAGAAGCACATTTCGGGATGCACGAGACACCGTACGTTTTGTACAATTCGGAC
AAAGATGCTACGGAGTCTAAAAACTCATGTTTCGTCGTTGTCAAAGACGATGGATTTTAT
CGTTGGAACAACAATAAGAGAATCTGGGACAAGGTTTTTCCGATCATTTGGAACTAAATC
ATTTATATTTTTCAAATGGTACCTTATGTTTGAGGCATCAGGAAAATGAATAAAGTAATT
TCCAAAAAAAAAATCATTTATATTTTTTATTACCAATTTTTTTGTTGAACAAATAACAAC
TATGCAAAATTAAAAAGATATTATAAGATGCTAGCATTATGTACACTGCTCCAATAATGT
TTGGCTTTTTCAATGTTCACACAAGTTTAGTGTGAACTTGCCAAACTCTATATATATTGA
TATAAAGACATATAAAATTTTTTTGATATAAAGCTAGTGGATGTTATCAAATTCTGTTAC
ATGCATCGTTAACGCAAATTCAACTTGTCATATCAAATGGAAAAACCAAAACTATATTCA
TTATAAATAGTGTTGTTTGACATTTTCACACAAATTTATATAACATATGTATATAAACTT
TTATTATATCTCTAAAATTTAAATTGAATTTTGATATTCTGATCTAAATGTTGACTTGAA
TTTTAGTTTAGTTTTGAAACATCTAAT

Last Updated on 06.12.2018 16:11