Sequences from confirmation amplicon


Confirmation sequences for line 339B09 and hit At5g52070 are shown.
Line ID 339B09
Locus At5g52070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21163491
Primers used for PCR AK44 - 8474, show primer details
   
Sequence Name 14-K105704-0022-339-B09C-AK44
GenBank Accession LN492507 [GenBank]
Sequencing primer gene specific primer, AK44: 5'-GCACATCCATATAGAGATTCCGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K105704-0022-339-B09C-AK44
GATTTCTGATGGCAGCCTTGTAATTAGATTGGTATAGCTGATCTCTCCATTTTTTTATGT
TAGCAAAGCAATATTTACAAAATTACTTCAAATCTCGTAGGAGCTTTAAGATTTCTTCTC
ATTATCTCTAGCAAGTTGAGTAATCTGCTAGTTCTAACTTAGATCCTCAACTCCTCTTCC
TTAATAGCACTCGTAGCATTGTCATCAGATTCACCAATATCTCCTCGTAACTCTGATGAT
GTTCACCTGTAGCTCATTATAGAATTCATCTTTAACGTTGTCATCATTTTACAGGATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCG
   
Sequence Name 30-K105705-0022-339-B09C-8409
GenBank Accession LN499720 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K105705-0022-339-B09C-8409
GAGCCATTTACATTGAATATATCCTGTAAAATGATGACAACGTTAAAGATGAATTCTATA
ATGAGCTACAGGTGAACATCATCAGAGTTACGAGGAGATATTGGTGAATCTGATGACAAT
GCTACGAGTGCTATTAAGGAAGAGGAGTTGAGGATCTAAGTTAGAACTAGCAGATTACTC
AACTTGCTAGAGATAATGAGAAGAAATCTTAAAGCTCCTACGAGATTTGAAGTAATTTTG
TAAATATTGCTTTGCTAACATAAAAAAATGGAGAGATCAGCTATACCAATCTAATTACAA
GGCTGCCATCAGAAATCCATTCAAGGACAAAGAGGAAGAGTCAATGATAAGAAAACGGAA
TCTCTTAGTGGATGTG

Last Updated on 06.12.2018 16:11