Sequences from confirmation amplicon


Confirmation sequences for line 339E12 and hit At4g18780 are shown.
Line ID 339E12
Locus At4g18780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10314983
Primers used for PCR NK58 - 8474, show primer details
   
Sequence Name 10-K103453-0022-339-E12C-NK58
GenBank Accession LN490615 [GenBank]
Sequencing primer gene specific primer, NK58: 5'-GTTATCCGCAAGGTTACAAATGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K103453-0022-339-E12C-NK58
TATCTGCGCAGTTCTACAATCTTTAAGTTTTATGAAACCATTTATCAAAACTATGCAGAT
TCGAAAGAGAAGGCGAGCAATCACAGCTTGCAGCTGTAGATTTCTTTGTTAGTACGGTAG
ATCCATTAAAGGAGCCACCTTTGATAACTGCAAACACGGTTCTTTCGATCCTCGCGCTTG
ATTATCCGGTGGATAAAGTCTCTTGCTATGTATCTGATGATGGTGCTGCAATGCTTTCGT
TTGAGTCTTTGGTTGAGACAGCAGATTTTGCTAGGAAATGGGTACCTTTCTGCAAAAAGT
ACTCCATCGAGCCACGAGCTCCCGAGTTTTACTTCTCGCTTAAAATCGATTACTTGAGGG
ATAAAGTTCAACCTTCTTTTGTGAAAGAACGTAGAGCCATGAAAGTAAGTAAGCAAGGGC
TTTAAAACCTTAAATTAATCTCATATAGCGTTAACCGATGATTGAATCGAAAACTTAATG
TCTAATTTCTCTTCTTGTAGAGAGATTATGAAGAGTTTAAAATAAGAATGAATGCTTTAG
TCGCCAAGGCTCAAAAGACACCAGAAGAAGGATGGACAATGCAAGATGGAACATCTTGGC
CCGGGAACAACACTACCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 26-K103454-0022-339-E12C-8409
GenBank Accession LN497883 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K103454-0022-339-E12C-8409
CGGGCCAGATGTTCATCTTGCATTGTCCATCCTTCTTCTGGTGTCTTTTGAGCCTTGGCG
ACTAAAGCATTCATTCTTATTTTAAACTCTTCATAATCTCTCTACAAGAAGAGAAATTAG
ACATTAAGTTTTCGATTCAATCATCGGTTAACGCTATATGAGATTAATTTAAGGTTTTAA
AGCCCTTGCTTACTTACTTTCATGGCTCTACGTTCTTTCACAAAAGAAGGTTGAACTTTA
TCCCTCAAGTAATCGATTTTAAGCGAGAAGTAAAACTCGGGAGCTCGTGGCTCGATGGAG
TACTTTTTGCAGAAAGGTACCCATTTCCTAGCAAAATCTGCTGTCTCAACCAAAGACTCA
AACGAAAGCATTGCAGCACCATCATCAGATACATAGCAAGAGACTTTATCCACCGGATAA
TCAAGCGCGAGGATCGAAAGAACCGTGTTTGCAGTTATCAAAGGTGGCTCCTTTAATGGA
TCTACCGTACTAACAAAGAAATCTACAGCTGCAAGCTGTGATTGCTCGCCTTCTCTTTCG
AATCTGCATAGTTTTGATAAATGGTTTCATAAAACTTAAAGATTGGTAGTAACTGCGCAG
ATTAAGCATATAACGGTTATAAAACATTTGTAACCTGCGGGATA

Last Updated on 06.12.2018 16:11