Sequences from confirmation amplicon


Confirmation sequences for line 339F09 and hit At3g09780 are shown.
Line ID 339F09
Locus At3g09780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3003039
Primers used for PCR 25hw - 8474, show primer details
   
Sequence Name 44-K112689-0022-339-F09C-25hw
GenBank Accession LN505237 [GenBank]
Sequencing primer gene specific primer, 25hw: 5'-CTTATAACCCCACACATCATTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K112689-0022-339-F09C-25hw
TATGATTATTGACTCCTATCTTACTTCTATTTTGTTACCTACTATATTATCCATTTCATA
TATGTTGACAAGTTGACAATGACGGTGAGCTCCACAACAACCAAACAAAAAAGTTTTTCT
TTTGTTTTAAAGCTCCAAAAAACTGACATGAAATCACTGGGATTAGATCTTATTGTCTCC
CATTAAGGCAAATTTCATTAGATTTTCCCTTTTTGGAAATAATTTTCTTTTCGATTTATC
AACTATAGTATATACCTTTTTTTACCCCCAAAAACCCAGGGATTATATATACGTAAATTA
TTTACATGCATGAATATGAATAGATACAGTAAATTAGTTGTTCAATAAAATATTTAAACT
TTTTGCTCTCATCTCCAATGAGGACGCCACCCACGTTCTCTGTTCGTTAGCCATCACACT
GTCAAATGGCAGCTTGTGTCAATCTTCTTCTTTCACAGTTCATGAACTTCACCTCCAATG
GAAACCCGTTGCTCTCTCCTCTTTCTCTCTCTCATTCTCCTGTATTTGCCCAAACCCGGA
TCCGGATTTGGATCCTCCGGTCCGATNGCGGCGTCGTTCGGCGGCTCTGCTTTCTTCTGC
GCCATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATT
   
Sequence Name 12-K112688-0022-339-F09C-8409
GenBank Accession LN491650 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K112688-0022-339-F09C-8409
TGGCGCAGAAGAAGCAGAGCCGCCGAACGACGCCGCAATCGGACCGGAGGATCCAAATCC
GGATCCGGGTTTGGGCAAATACAGGAGAATGAGAGAGAGAAAGAGGAGAGAGCAACGGGT
TTCCATTGGAGGTGAAGTTCATGAACTGTGAAAGAAGAAGATTGACACAAGCTGCCATTT
GACAGTGTGATGGCTAACGAACAGAGAACGTGGGTGGCGTCCTCATTGGAGATGAGAGCA
AAAAGTTTAAATATTTTATTGAACAACTAATTTACTGTATCTATTCATATTCATGCATGT
AAATAATTTACGTATATATAATCCCTGGGTTTTTGGGGGTAAAAAAAGGTATATACTATA
GTTGATAAATCGAAAAGAAAATTATTTCCAAAAAGGGAAAATCTAATGAAATTTGCCTTA
ATGGGAGACAATAAGATCTAATCCCAGTGATTTCATGTCAGTTTTTTGGAGCTTTAAAAC
AAAAGAAAAACTTTTTTGTTTGGTTGTTGTGGAGCTCACCGTCATTGTCAACTTGTCAAC
ATATATGAAATGGATAATATAGTAGGTAACAAAATAGAAGTAAGATAGGAGTCAATAATT
CATAATTTTTCATGAAATTTTAAATTATTGAGGAAATGATGTTGGG

Last Updated on 06.12.2018 16:11